Any heat treatment technology must begin with the process of austenization, during which the holding time at austenite region is one of the critical parameters. The effect of austenization treatment holding time on the martensitic transformation in the 30CrNi3MoV ultra-high-strength steel was investigated by means of dilatometric measurements and microstructural observations. The results showed that extending the holding time at 900 oC won’t cause obvious coarsening of austenite grains due to the dragging effect of vanadium solute atoms adsorbed at austenite grain boundaries. Martensite microstructures obtained in the 30CrNi3MoV samples that were heat treated with different holding time varied a little. The austenization holding time has obvious influence on the Ms of the 30CrNi3MoV steel, because it simultaneously affect the size of prior austenite grains and the configuration of dislocation defects.