Highly Efficient Synthesis of Silicon-Containing (R)-Ketone-Cyanohydrin Catalyzed by (R)-Hydroxynitrilase Lyase from Prunus Domestic

Sen Lin Liu

doi:10.4028/www.scientific.net/AMR.233-235.1494

Paper Titles

Photodegradation of Toluene by TiO₂ Nanoparticles by Flame CVD Process
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Study on Preparation of Dissolving Grade Pulp and its Kinetics of Prehydrolysis
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Degradation of 4-Chlorophenol in Wastewater Using Nanometer TiO₂ Film under UV Light Irradiation
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Stability of the Catalyst for C₄ Alkenes to Propylene and Ethylene
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Highly Efficient Synthesis of Silicon-Containing (R)-Ketone-Cyanohydrin Catalyzed by (R)-Hydroxynitrilase Lyase from Prunus Domestic
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Corrosion Behavior of Sealed Films on Anodized Al Alloy
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Influence of Preparation Conditions on the Acidity of WO₃/ZrO₂ Solid Superacid
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Synthesis of Mesostructured Materials of Different Heteroatoms and Their Catalytic Performances for Styrene Epoxidation
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Sintering Kinetics of Hydroxyapatite Prepared by Chemical Coprecipitation Method
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Highly Efficient Synthesis of Silicon-Containing (R)-Ketone-Cyanohydrin Catalyzed by (R)-Hydroxynitrilase Lyase from Prunus Domestic

Abstract:

The synthesis of chiral silicon-containing (R)-ketone-cyanohydrin by enantioselective transcyanation of acetyltrimethylsilane with acetone cyanohydrin was efficiently carried out using defatted Prunus domestic seed meal as (R)-hydroxynitrilase lyase for the first time. Under the optimal conditions including a reaction temperature of 25-35°C and a buffer pH of 5.0-5.4, both acetyltrimethylsilane conversion and enantiomeric excess of the product were above 99%. In contrast, this enzyme did not accepted its carbon counterpart 3,3-dimethyl-2-butanone as substrate. These results demonstrated that the silicon atom in substrate served as a more effective atom than the carbon atom to enhance the activity of the enzyme.