Highly Efficient Synthesis of Silicon-Containing (R)-Ketone-Cyanohydrin Catalyzed by (R)-Hydroxynitrilase Lyase from Prunus Domestic

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The synthesis of chiral silicon-containing (R)-ketone-cyanohydrin by enantioselective transcyanation of acetyltrimethylsilane with acetone cyanohydrin was efficiently carried out using defatted Prunus domestic seed meal as (R)-hydroxynitrilase lyase for the first time. Under the optimal conditions including a reaction temperature of 25-35°C and a buffer pH of 5.0-5.4, both acetyltrimethylsilane conversion and enantiomeric excess of the product were above 99%. In contrast, this enzyme did not accepted its carbon counterpart 3,3-dimethyl-2-butanone as substrate. These results demonstrated that the silicon atom in substrate served as a more effective atom than the carbon atom to enhance the activity of the enzyme.

Info:

Periodical:

Advanced Materials Research (Volumes 233-235)

Edited by:

Zhong Cao, Lixian Sun, Xueqiang Cao, Yinghe He

Pages:

1494-1497

DOI:

10.4028/www.scientific.net/AMR.233-235.1494

Citation:

S. L. Liu "Highly Efficient Synthesis of Silicon-Containing (R)-Ketone-Cyanohydrin Catalyzed by (R)-Hydroxynitrilase Lyase from Prunus Domestic", Advanced Materials Research, Vols. 233-235, pp. 1494-1497, 2011

Online since:

May 2011

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$35.00

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