Three-bending experiments were performed on RC beams strengthened with prestressed CFRP under static and cycle loading. Prestressed CFRP obviously increases the load-carrying capacity and fatigue life of strengthened RC beam. And it greatly increases the flexural stiffness of strengthened beam. The failure modes of the beams go through concrete cracking, CFRP debonding from concrete and beam fractured. The vertical flexibility history at midspan is decomposed into three stages. And then fatigue damage is defined by flexural stiffness and its evolvement shows three stages of nucleation, growth and mutation.