Forged Ti-6Al-4V welded structure by electronic beam welding (EBW) as integrally bladed disk (blisk) structure in advanced aero-engine has been widely applied. It is necessary to analyze microstructure and mechanical properties of Ti-6Al-4V welded joints by EBW for failure analysis and structure design of blisk. Firstly the microstructure and mechanical properties of forged Ti-6Al-4V welded joints was focused on. Grains in the weld zone become coarse and large gradient organization structure appears in the heat affected zone (HAZ), which presents significant local heterogeneity. Microhardness of the weld zone is about 20% higher than that of the base metal. The size of different region of the welded joints was estimated. Then static tensile test of three different specimens were carried on. Experiment results show that the tensile and yield strength of welded joints are not less than that of the base metal. Finally the empirical relationship between strength and hardness of Ti-6Al-4V alloy is established. Tensile strength of the weld zone and the base metal were estimated. Compared to experiment data, the deviation is 3.56%, 0.097% respectively.