The push and pull force of Substitute Astronauts (SA) were assessed by analyses with Operation Force Measuring System (OFMS) in laboratory. Experiment comprised 3D dimensional (3D) measurements of hand force during operating in two different modes of manual control when SA grasp the handle of OFMS at comfortable position. 8 selected SA took part in experiments that were required to wear spacesuit. The investigation revealed that the longitudinal force components are often considerable amplified when the handle was pushed and pulled in the horizontal/perpendicular direction. In some cases, it equals to or exceeds the force in the direction of movement. The results demonstrate, when SA push and pull the handle at comfortable position by single hand, the respective sort orders of cumulative peak force in 3D directions are Fx>Fy>Fz(horizontal), Fy>Fz>Fx (vertical), Fz>Fy>Fx (lateral), the highest values of force occurs while pushing and pulling in the longitudinal direction.