With the demands of quantity and quality for water are increasing rapidly and a lot of wastewater treatment processes are difficult to be used abroad. Therefore, how to enhance the efficiency and to reduce consumption is the key to solve these problems. For purpose of enhancing mass transfer in the system, the reactor was modified with the structure characteristic of opposite folded plate. and the system with the characteristic of multi-phase anaerobe of compartments longitudinally down reactor and two-double phase of sludge- biomembrane in every compartment. The results was when the HRT≥6h and the sludge retention time (SRT) was controlled 120 days, the pilot-scale opposite folded plate hybrid anaerobic reactor (OFPHAR) was Two-phase Anaerobic system. The trial results indicated that the optimal HRT was 6h at (25±2) °C, and the corresponding removal rates of COD, TP and TN removal rates were 78.58%, 35.15%, 39.17% respectively.