Novel DNA Electrochemical Biosensor Using Anthraquinone-2-Sulfonic Acid Sodium Salt as Hybridization Indicator

Abstract:

A novel DNA biosensor based on layer-by-layer self-assembled multi-walled carbon nanotubes (MWNTs) functionalized with a mercapto group (SH-MWNTs) and gold nano-particles (GNPs) was presented, where anthraquinone-2-sulfonic acid sodium salt (AQMS) was used as hybridization indicator. The differential pulse voltammetry responses demonstrated that this DNA/GNPs/SH-MWCNTs/Au biosensor was enabled to specifically detect the single-base mismatch DNA sequence in phosphate buffer solution with pH 7.4 containing 0.3 mol/L Na+ and 1.0 mmol/L AQMS. The result showed that when the target DNA concentration was 1.0×10-10 to 1.6×10-5 mol/L, the cathodic peak current of Au electrode system with AQMS as indicator was linearly related to complementary NDA concentration, and the detection limit was about 3.82×10-11 mol/L and had good stability and specificity.

• Periodical: Advanced Materials Research (Volume 298)
• Pages: 56-62
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.298.56
• Online since: July 2011
• Authors: Mei Ma, Xiao Ping Fan, Zhao Dai, Xiao Qing Wang, Qing Yin Zhang
• Keywords: Anthraquinone, DNA Biosensor, Electrochemical (EC), Hybridization, Indicators

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