Endothelialisation and Cell Retention on Gelatin Chitosan-Coated Electrospun Polyurethane, Poly (Lactide-Co-Glycolide) and Collagen-Coated Pericardium
Arterial bypass and heart valve replacements are two of the most common surgical treatments in cardiovascular surgery today. Currently, artificial materials are used as substitute for these cardiac tissues. However, these foreign materials do not have the ability to grow, repair or remodel and are thrombogenic, leading to stenosis. With the aid of tissue engineering, it is possible to develop functional identical copies of healthy heart valves and arteries, which are biocompatible. Although much effort has been made into this area, there are still inconsistencies with respect to endothelialisation and cell retention on synthetic biological grafts. These variations may be attributed to differences in factors such as cell seeding density, incubation periods and effects of shear stress. In this study, we have compared the endothelialisation and cell retention between gelain chitosan-coated electrospun polyurethane (PU), poly (lactide-co-glycolide) (PGA/PLA) and collagen-coated pericardium. Endothelial cells adhered to all of the materials as early as 1–day post seeding. After 7-day of seeding, the coverage on PU was almost 45% and that on PGA/PLA was about 25% and the least was on collagen-coated pericardium of approximately 15%. It was observed that the PU showed superior cell coverage and cell retention in comparison to the PGA/PLA and collagen-coated pericardium.
John Bell, Cheng Yan, Lin Ye and Liangchi Zhang
S. Patel et al., "Endothelialisation and Cell Retention on Gelatin Chitosan-Coated Electrospun Polyurethane, Poly (Lactide-Co-Glycolide) and Collagen-Coated Pericardium", Advanced Materials Research, Vol. 32, pp. 233-236, 2008