The paper introduces the status of rolling bearing life testing machine in China first. Then, the paper mainly focuses on the design of vibration signal data acquisition system for rolling bearing life testing machine. The system is developed for a rolling bearing manufacturer based on Labview with perfect functions according to the requirement of the customer. The rolling bearing manufacturer can set up different measurement time and time interval for data record to carry out test according to the requirement of its customers. The developed vibration signal acquisition system is not subject to testing machine, which can run only that the sensors are installed on the measurement locations of testing machine. The developed system can be used for different rolling bearing life testing machine, so it has good application perspective in future.