Microbial Fermentation Extraction Processing of Protein from Soybean

Abstract:

Although hexane extraction of soybean oil has the high recovery，residual hexane in oil is noxious for consumer health. Crew pressing and extruding-expelling extraction processes denature protein to render functional properties poor for food and meal use. This processing employed microbial fermentation to extract protein from full fat soy flour so that denaturation degree of protein reduced. The results showed that low temperature flaking and extrusing soy flakes was beneficial to improving protein extraction, after optimizing the fermentation conditions with the Response Surface Methodology (RSM), total protein extraction yield achieved the highest value - 86.66% at optimal fermentation conditions - 6.6% extruded full fat soy flour, 5.4% inoculation volume, the initial pH8.1. These advances make the microbial fermentation extraction process of protein from soybeans attractive channel as the environmentally – friendly technology.

• Periodical: Advanced Materials Research (Volume 345)
• Pages: 168-173
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.345.168
• Online since: September 2011
• Authors: Hai Bo Wu, Lian Zhou Jiang, Yang Li, Hai Tao Wu
• Keywords: Full Fat Soy Flour, Microbial Fermentation, Total Protein Extraction Yield

References

[1] Johnson LA, Recovery of fats and oils from plant and animal sources, In: Wan PJ, Far W(eds) Introduction to fats and oils. AOCS Press. champaign. (2000) pp.108-135.

[2] Nelson LL, Wijeratne WB, Yeh SW, Wei TM, Wei LS, Dry extrusion as an aid to mechanical expelling of oil from soybeans, Am Oil Chem Soc, Vol. 64 (1987): 1341–1347.

DOI: 10.1007/bf02540794

[3] Heywood AA, Myers DJ, Bailey TB, Johnson LA, Functional properties of extruded-expelled soybean flours from valueenhanced soybeans, Am Oil Chem Soc , Vol. 79 (2002): 699–702.

DOI: 10.1007/s11746-002-0545-z

[4] Heywood AA, Myers DJ, Bailey TB, Johnson LA, Functional properties of low-fat soy flour produced by and extrusionexpelling system, Am Oil Chem Soc, Vol. 79 (2002): 1249– 1253.

DOI: 10.1007/s11746-002-0635-y

[5] LiYang, Lianzhou Jiang, Zhaoguo Zhang, Haibo Wu, Effect of Cellulose Degradation on Soybean Oil Extraction Yield through Extrusion and Expansion Processing, Transaction of the Chinese society for agricultural machinery Vol. 02 (31) (2010).

[6] LiuYang, Lianzhou Jiang, YangLi, Haibo Wu, Ultrasonic preirradiation effect upon aqueous enzymatic oil extraction from soybean, China oil Vol. 34 (12) (2009): 10-14.

[7] Yang Li, Lianzhou Jiang, Haibo Wu, JingXu, Effect of extrusion and expansion parameters on recovery of soybean protein by aqueous enzymatic method, Journal of the Chinese Cereals and Oils Association Vol. 12 (24) (2009): 47-51.