Blue Fluorescent Quantum Dots-Based Probe for Detection of Influenza B Virus

Jun Lin Wen; Dai Gui He; Su Qing Zhao

doi:10.4028/www.scientific.net/AMR.365.18

Paper Titles

Preface and Organizing Committee

Prediction of the Deterioration Depth of Concrete by Accelerating Calcium Leaching Test
p.3

Factors Affecting the Performance of Molten Salt Heat Receiver
p.8

Expressions of Parts Information in Collaborative Design
p.14

Blue Fluorescent Quantum Dots-Based Probe for Detection of Influenza B Virus
p.18

Propagation of SH Wave in Composites with Parallel Cylindrical Nanoholes
p.23

Finite Element Simulation and Respond Surface Optimization on Stretch Bending of Square Tube Aluminum Profile
p.28

Classification of Fine Recycled Aggregate Used in Asphalt Concrete
p.33

Texture Analysis of Turned Surface Images Using Grey Level Co-Occurrence Technique
p.38

HomeAdvanced Materials ResearchAdvanced Materials Research Vol. 365Blue Fluorescent Quantum Dots-Based Probe for...

Blue Fluorescent Quantum Dots-Based Probe for Detection of Influenza B Virus

Abstract:

Influenza is a common acute viral disease that has caused massive harm to human, so a rapid pathogen-detecting method is required for monitoring the status of the disease. Quantum dots are promising emerging fluorescent probe for diagnostic assay. In this work, water soluble ZnO quantum dots were covalently conjugated to rabbit anti-chicken IgY antibody to prepare the quantum dots-linked secondary antibody. The conjugate was characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and fluorescence spectrophotometer. Fluorescent quantum dots-based immunosorbent assay was conducted to determine the concentration of influenza B virus. We found that rabbit anti-chicken IgY antibody augmented its molecular weight while coupled to ZnO quantum dots, but the emission spectrum didn’t change. More importantly, the luminescence intensity of QDs correlated to viral concentration in a linear relationship. These results may suggest a potential new approach for quantifying virus, pathogenic bacteria and toxic molecule.

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Periodical:

Advanced Materials Research (Volume 365)

Pages:

18-22

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.365.18

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Online since:

October 2011

Authors:

Jun Lin Wen, Dai Gui He, Su Qing Zhao

Keywords:

Egg Yolk Antibody (IgY), Fluorescent Probe, Influenza B Virus, Quantum Dot (QD)

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