Extraction of Collagen from Fish Scales with Papain under Ultrasonic Pretreatment
The extraction of collagen from fish scales with papain under ultrasonic pretreatment was conducted in this paper. The effects of the ultrasonic pretreatment time, the ratio of papain to fish scales, the extraction temperature and the extraction time on the extraction rate of collagen were investigated by single factor experiment and orthogonal experiment, respectively. The results showed that the optimum conditions for collagen extraction from fish scales were: ultrasonic pretreatment time 4min, ratio of papain to fish scales 4%, temperature 60°C and extraction time 5h. Under the optimum conditions the extraction rate of collagen reached 90.7%.
Helen Zhang and David Jin
J. N. Zeng et al., "Extraction of Collagen from Fish Scales with Papain under Ultrasonic Pretreatment", Advanced Materials Research, Vol. 366, pp. 421-424, 2012