In Vitro Antitumor Activities of Low Molecular Sea Cucumber Stichopus japonicus Peptides Sequentially Hydrolyzed by Proteases

Xiao Qiu Zhou; Chang Hai Wang; Ai Li Jiang

doi:10.4028/www.scientific.net/AMR.393-395.1259

Paper Titles

Ferric Nitrate Suppresses Internal Phosphorus Loading in Municipal River
p.1242

Game-Theoretic Analysis of Ecological Restoration
p.1247

Graft Modification of Collagen with Acrylonitrile in Concentrated Zinc Chloride Aqueous Solution
p.1251

Hydrothermal Treatment and its Influence on the Structure of Nitrogen Doped Titania
p.1255

In Vitro Antitumor Activities of Low Molecular Sea Cucumber Stichopus japonicus Peptides Sequentially Hydrolyzed by Proteases
p.1259

Influences to Forest Fire Occurrence of Climate Change: A Study in Ta He Forestry Bureau in Great Xing’an Mountain
p.1263

Kinetics and Thermodynamics of Adsorption of Copper Ions from Aqueous Solutions onto Carboxymethylcellulose Sulfate
p.1268

Mechanism Study on Forming Flake Silver Tin-Oxide Composite Powder
p.1273

Microencapsulation of Styrene/Epoxydiacrylate via In Situ Polymerization of Melamine-Formaldehyde
p.1279

HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 393-395In Vitro Antitumor Activities of Low Molecular Sea...

In Vitro Antitumor Activities of Low Molecular Sea Cucumber Stichopus japonicus Peptides Sequentially Hydrolyzed by Proteases

Abstract:

The sea cucumber (Stichopus japonicus) was continuously hydrolyzed by pepsin, trypsin to get sea cucumber peptides (SCP), and low molecular peptides (LSCP), MW < 3000 Da, were collected through an ultrafiltration membrane with a molecular weight cut-off of 3000 Da Two main fractions, LSCP-1 and LSCP-2 were obtained by one step gel filtration column chromatography. Furthermore, the antitumor activities, in vitro, of LSCP-1 and LSCP-2 fractions were evaluated by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay. LSCP-2 showed significantly antitumor activity against the SGC-7901 and MCF-7 cell lines, LSCP-1 showed significantly antitumor activity only against SGC-7901 cell line, and two fractions did not show activity against A549 cell line. Overall, this study indicated that LSCP posseses potential to treat gastric cancer and breast cancer.

You might also be interested in these eBooks

Biotechnology, Chemical and Materials Engineering

View Preview

Info:

Periodical:

Advanced Materials Research (Volumes 393-395)

Pages:

1259-1262

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.393-395.1259

Citation:

Cite this paper

Online since:

November 2011

Authors:

Xiao Qiu Zhou, Chang Hai Wang, Ai Li Jiang

Keywords:

Antitumor Activities, Peptide, Stichopus japonicus, Ultrafiltration (UF)

Export:

RIS, BibTeX

Price:

Permissions CCC:

Request Permissions

Permissions PLS:

Request Permissions

Сopyright:

Citation:

References

[1] M. Saito, N. Kunisaki and N. Urano: Food Sci. vol. 67, (2002), p.1319.

Google Scholar

[2] A.M. Rafiuddin, U. Venkateshwarlu and R. Jayakumar: Biomater. vol. 25, (2004), pp.2585-94.

Google Scholar

[3] Y. L. Chen, S. Z. Lin, J.Y. Chang, et al: Biochem. Pharmacol. vol. 72, (2006), p.308–319.

Google Scholar

[4] T. L Simmons, E. Andrianasolo, K. McPhail, et al: Mol. Cancer. Ther. vol. 4, (2005), pp.333-342.

Google Scholar

[5] B.O. Leng, D.L. Xiao, X.C. Qing: FEBS Lett. Vol. 579, (2005), pp.1187-1190.

Google Scholar

[6] B. Zhang, W.T. Wu: Chin. J. Nat. Med. vol. 4, (2006), pp.230-233.

Google Scholar

[7] X.D. Liu, L. Qiu, Q. Wu: J. Xiamen Univ. (Nat. Sci. ) vol. 43, (2004), pp.432-435.

Google Scholar

[8] G.R. Pettit, Y. Kamano, C.L. Herald, et al: J. Am. Chem. Soc. vol. 109, (1987), pp.6883-6885.

Google Scholar

[9] Y.H. Zhao, B.F. Li, Z.Y. Liu, et al: Process Biochem. Vol. 42, (2007), pp.1586-1591.

Google Scholar

[10] Y.H. Zhao, B.F. Li, S.Y. Dong, et al: Peptides vol. 30, (2009), pp.1028-1033.

Google Scholar

[11] J.F. Wang, Y.M. Wang, Q.J. Tang, et al: J. Ocean Univ. China vol. 9, (2010), pp.94-98.

Google Scholar