Effect of Succinylation on Aqueous Enzyme-Assisted Extraction of Oil from Soybean

Abstract:

In order to improve the total soybean oil rate and reduce the amount of emulsion in enzyme-assisted aqueous extraction, succinic anhydride was used to modify the protein in the enzymatic hydrolysis process. The amount of the enzyme, liquid to material ratio, additive amount of succinic anhydride, hydrolysis time and the influence of modification time on the total oil rate were studied in this experiment. Response surface analysis method was employed to optimize experimental parameters. The total oil extraction rate was 94.49 ± 0.98% when the optimum parameters were chosen as follows: enzyme additive amount 2.83%, liquid to material ratio 6.34, additive amount of succinic anhydride 2.95%, hydrolysis time 2.53h and modification time 2.48h.

• Periodical: Advanced Materials Research (Volumes 393-395)
• Pages: 696-703
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.393-395.696
• Online since: November 2011
• Authors: Wen Liu, Lian Zhou Jiang, Yang Li, Xiao Nan Sui
• Keywords: Enzyme-Assisted Aqueous Extraction, Modification, Soybeans, Succinylation

References

[1] A.I. Nelson, W.B. Wijeratne, S.W. Yeh, T.M. Wie and L.S. Wei. 1987. Dry extrusion as an aid to mechanical expelling of oil from soybeans. Journal of the American Oil Chemists Society. 64(9), 1341-1347.

DOI: 10.1007/bf02540794

[2] M. Guderjan, P. Elez-Martinez and D. Knorr. 2007. Application of pulsed electric fields at oil yield and content of functional food ingredients at the production of rapeseed oil. Innovative Food Science and Emerging Technologies. 8, 55-62.

DOI: 10.1016/j.ifset.2006.07.001

[3] J. Sinero, H. Dominguez, M.J. Nunez and J.M. Lema. 1998. Optimization of the Enzymatic Treatment during Aqueous Oil Extraction from Sunflower Seeds. Food Chem. 61, 467-474.

DOI: 10.1016/s0308-8146(97)00080-0

[4] B.P. Lamsal and L.A. Johnson. 2007. Separating oil from Aqueous Extraction Fractions of soybean. Journal of the American oil Chemist's society. 84 (8), 785-792.

DOI: 10.1007/s11746-007-1090-0

[5] O. Valentova, Z. Novotna, Z. Svoboda, W. Schwartz, J. Kas. 2000. Microwave heating and c-irradiation treatment of rapeseed (Brassica napus). Journal of Food Lipids. 7, 237-245.

DOI: 10.1111/j.1745-4522.2000.tb00175.x

[6] S.B. Zhang, Z. Wang, S.Y. Xu. 2007. Optimization of the Aqueous Enzymatic Extraction of Rapeseed Oil and Protein Hydrolysates. J Amer Oil Chem Soc. 84, 97-105.

DOI: 10.1007/s11746-006-1004-6

[7] P. SunStudy on the technology of soy protein modification. Food Research and Development. 2005, 26(5): 115-117.

[8] Thompson L U, Reyes E S. Modification of heat coagulatedwhey protein concentrates by succinylation [J]. Dairy Science. 1980, 63(5): 715- 721.

DOI: 10.3168/jds.s0022-0302(80)82998-5

[9] Gruener I , IsmondM A H. Effects of acetylation and succinylation on the functional properties of the canola 12S globulin. Food Chemistry. 1997, 60(4): 513- 520.

DOI: 10.1016/s0308-8146(97)00016-2

[10] G.H. Zhang, G.C. Wang, Z.D. Chen. Effects of Modification on Functionalities and Structure of Corn Gluten Protein(II)Acylation. Journal of the Chinese Cereals and Oils Association, 2000, 15(4): 14- 17.

[11] Lawal O S, Adebowale K O. Effect of acetylation and succinylation on solubility profile, water absorption capacity, oil absorption capacity and emulsifying properties of mucunabean (Mucuna pruriens) protein concentrate. Nahrung /Food, 2004, 48(2): 129- 136.

DOI: 10.1002/food.200300384

[12] Mohamed A, Biresaw G, Xu J Y. Oats protein isolate: Therma, lrheologica, surface and functional properties [J]. Food Research Internationa, l 2009, 42(1): 107- 114.

DOI: 10.1016/j.foodres.2008.10.011

[13] Mirmoghtadaie L, KadivarM, Shahedi M. Effects of succinylation and deamidation on functional properties of oat protein isolate. Food Chemistry, 2009, 114(1): 127- 131.

DOI: 10.1016/j.foodchem.2008.09.025

[14] M. Guderjan, P. Elez-Martinez and D. Knorr. 2007. Application of pulsed electric fields at oil yield and content of functional food ingredients at the production of rapeseed oil. Innovative Food Science and Emerging Technologies. 8, 55-62.

DOI: 10.1016/j.ifset.2006.07.001

[15] S. Shah, A. Sharma and M.N. Gupta. 2005. Extraction of oil from Jatropha curcas L. seed kernels by combination of ultrasonication and aqueous enzymatic oil extraction. Bioresource Technology. 96 (1), 121-123.

DOI: 10.1016/j.biortech.2004.02.026

[16] O. Valentova, Z. Novotna, Z. Svoboda, W. Schwartz and J. Kas. 2000. Microwave heating and c-irradiation treatment of rapeseed (Brassica napus). Journal of Food Lipids. 7, 237-245.

DOI: 10.1111/j.1745-4522.2000.tb00175.x