An Improved Chronoamperometry for Electrochemical Real-Time PCR Measurement

Abstract:

Electrochemical real-time PCR (EC-rtPCR) overcomes several shortcomings of fluorescence-based real-time PCR. But traditional electrochemical method has some limitations which reduce the accuracy and efficiency of testing. To overcome the disadvantages of chronoamperometry (CA) we report a novel electrochemical method where a peak current is quickly generated for the current vs. time curve by changing the waveform of voltage excitation in the working electrode. In particular, we derived a mathematical model to illustrate the principle of this method and it can also be used to demonstrate that the peak current is linear with regards to the concentration of the target substance. Moreover, we developed a device with an improved electrochemical circuit to generate the voltage excitation and detect the peak automatically. Finally, the device was used to study the electrochemical behavior of K3[Fe(CN)6]. It’s shown that the method has a better signal to noise ratio and higher sensitivity than chronoamperometry. The obtained peak current is linear with regards to the concentration of the target substance.

• Periodical: Advanced Materials Research (Volumes 396-398)
• Pages: 110-114
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.396-398.110
• Online since: November 2011
• Authors: Xu Hai Chen, Min Du
• Keywords: Chronoamperometry, Cottrell Equation, Electrochemical Device, Electrochemical Real-Time PCR

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