Application of Response Surface Methodology to Optimise Preparation High Antioxidant Activity Product from Pinctada fucata Muscle

Abstract:

The high antioxidant activity product was isolated from Pinctada fucata muscle by a method of Alcalase hydrolysis. Optimization using response surface methodology was performed and 3D response surfaces were plotted from the mathematical model. The results indicated that the optimal extraction conditions were as follows: pH 7.0, temperature 61°C, E/S 3.01%, hydrolysis time 3h, under the conditions of hydrolysis products of the DPPH radical scavenging rate of 67.3% and the degree of hydrolysis of 31.2%, which was in good agreement with the predicted model value.

• Periodical: Advanced Materials Research (Volumes 396-398)
• Pages: 1341-1348
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.396-398.1341
• Online since: November 2011
• Authors: Yan Yan Wu, Lai Hao Li, Zhen Hua Duan, Xian Qing Yang, Jun Shang, Sheng Jun Chen
• Keywords: Antioxidant Activity, Enzymatic Hydrolysis, Pinctada fucata Muscle, Response Surface Method (RSM)

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