Study on Screening of Glutathione over-Production Strain and its Fermentation Conditions

Jie Gang; Li Feng Shan; Hong Yu Bu

doi:10.4028/www.scientific.net/AMR.396-398.1657

Paper Titles

Effect of Two Mild Alkaline Oxidative Pretreaments of Rice Straw on Enzymatic Hydrolysis
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Kinetic Study of Titanium-Modified Phenolic Resin Curing Process by DSC Analysis
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Antioxidant Properties of Cashew Apple Wine (CAW)
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Effects of Milk Composition Proportioning on the Texture Properties of Rennet Gels Produced by Reconstituted Milk Powder
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Study on Screening of Glutathione over-Production Strain and its Fermentation Conditions
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Optimization of L-Arabinose Hydrolization from Corn Fibers Using Response Surface Methodology
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Synthesis of Super-High Molecular Weight Polyacrylamides and their Flocculation Properties
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The Distribution and Pasting Properties of Starches in Fresh Sweet Potato (Ipomoea batatas Lam.) Tuber
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Metabonomics and the Research of Traditional Chinese Medicine
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Study on Screening of Glutathione over-Production Strain and its Fermentation Conditions

Abstract:

This study was to screen strains for glutathione over-production. A high GSH-producing strain HSJB1 was isolated from soil.According to the morphological, physiological and biochemical characteristics of cells, this strain was primarily identified as Saccharomyces cerevisiae. The glutathion fermentation conditions of this strain by shake flask culture were studied.The optimal fermentation medium was as follows: glucose 20g/L, (NH4)2SO4 5g/L, peptone 12.5 g/L, KH2PO4 9.0 g/L, MgSO4•7H2O 1.0 g/L, NaCl 0.2 g/L, ZnSO4•H2O 0.01 g/L. The optimal culture conditions were as follows :pH 5，broth quantity 30ml/250ml, temperature 28°C，inoculum 10%. Under the optimal conditions, the glutathione productivity and biomass were 53.60 mg/L and 3.8 g/L, respectively, which were 11.2% and 11.8% higher than those in the original conditions, respectively.