Construction of a Novel Recombinant Escherichia coli Strain Capable of Producing 1,3–propanediol

Abstract:

1, 3-propanediol (1, 3-PD) is biologically synthesized by glycerol dehydratase (GDHt) and 1, 3-propanediol dehydrogenase (PDOR). In present study, the gldABC gene, encoding GDHt from Klebsiella pneumoniae and the yqhD gene, encoding PDOR isoenzyme from E.coli BL21 were cloned and co-expressed in E.coli JM109 using plasmid pSE380. The over-expressed recombinant enzymes were purified by nickel-chelate chromatography combined with gel filtration to study the properties. Optimal temperature and pH of recombinant GDHt with specific activity of 85.8 U/mg were 45 °C and 9.0; and optimal temperature and pH of recombinant YqhD with specific activity of 80.0 U/mg were 37 °C, 7.0. The microbial conversion of 1,3-PD from glycerol by this recombinant E. coli strain was studied and the production of 1,3-PD was about 28.0 g/l.

• Periodical: Advanced Materials Research (Volumes 396-398)
• Pages: 2499-2502
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.396-398.2499
• Online since: November 2011
• Authors: Xiang Hui Qi, Qi Guo, Yu Tuo Wei, Hong Xu, Ri Bo Huang
• Keywords: 1,3-Propanediol Dehydrogenase Isoenzyme, Co-Expression, Glycerol Dehydratase

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