Effects of Linoleic Acid on Hepatopancreatic Cell Proliferation of Prawn, Penaeus vannamei

Hong Wei Wang; Duan Bo Cai; Kai Ming Li; Hai Ming Xu; Ming Duan

doi:10.4028/www.scientific.net/AMR.403-408.1368

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 403-408Effects of Linoleic Acid on Hepatopancreatic Cell...

Effects of Linoleic Acid on Hepatopancreatic Cell Proliferation of Prawn, Penaeus vannamei

Abstract:

The effects of linoleic acid on hepatopancreatic cell culture of the kuruma prawn, Penaeus vannamei were conducted. The culture system consists of 199 media (M 199) supplemented with 0.060 mol/L NaCl, 1.011g/L glucose, 1000 UI/ml penicillin, 1000 μg/ml treptomycin, 20% heat inactivated fetal calf serum (FCS) for primary cells and 10 % for subculture cells. The content of phosphorus in cultured cells was measured. The results show that the growth condition of cultured hepatopancreas cells in P. vannamei was significantly improved by added 160 μmol/L linoleic acid.