Optimization of the Components of Medium Producing Dextran from Immobilized Cells of Leuconostoc mesenteroides

Qing Wang; Xiao Wang; Qing Song Zou; Yuan Yuan Pu; Shan Chen

doi:10.4028/www.scientific.net/AMR.418-420.212

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 418-420Optimization of the Components of Medium Producing...

Optimization of the Components of Medium Producing Dextran from Immobilized Cells of Leuconostoc mesenteroides

Abstract:

The cells of Leuconostoc mesenteroides were immobilized for dextran producing and response surface methodology based on experimental designs were applied to optimize the medium for maximum yield. Eight different medium components were examined for their significance on dextran production using Plackett-Burman factorial design. Na₂HPO₄, CaCl₂ and sodium citrate were found to have significant effect on dextran production. The combined effect of these nutrients on dextran production was studied using central composite design. The optimal concentration of variables for maximum dextran production were 0.245 g/100mL of Na₂HPO₄, 0.05 g/100mL of CaCl₂ , 0.2084 g/100mL of sodium citrate. The maximum concentration of dextran obtained at 24 h by predicted model was 1.88045 mg/mL that was in perfect agreement with the experimental determined value 1.85395 mg/mL. Dextran yield was over 376% higher as compared to original medium which gave 0.38956 mg/mL of dextran.

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Materials Processing Technology, ICAMMP2011

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Periodical:

Advanced Materials Research (Volumes 418-420)

Pages:

212-216

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.418-420.212

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Online since:

December 2011

Authors:

Qing Wang, Xiao Wang, Qing Song Zou, Yuan Yuan Pu, Shan Chen

Keywords:

Dextran, Immobilized Cell, Optimization, Response Surface Methodology (RSM)

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