Study of Collagen Assemble into Gel In Situ Used as Tissue Engineering Scaffold

Abstract:

Collagen is a kind of protein which can self-assemble into collagen gel in vitro at 37°C in neutral pH. In this work, collagen is extracted from bovine tendon and characterized by amino acid analysis and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). When pH of collagen solution is adjusted to 6.6~6.8 and incubated at 37°C for an hour, collagen molecular can be assembling into gel. As a result, collagen fibers in gel observed form electron microscopic (SEM and TEM) look like natural collagen fiber with cross-grain, which is caused by collage molecular assembly behavior. Then mouse embryonic fibroblasts (3T3 cells) are seeded in the gel. Within a period, state of 3T3 cell living in the 3D gel scaffold is observed. As a result, when collagen solution is mixed with 3T3 cells and incubated in vitro, the gel is formed and provides a three-dimensional space for 3T3 cells proliferation at the same time. This study provides a method to make tissue engineering scaffold with cells in situ and predicts its application in soft tissue defect healing.

• Periodical: Advanced Materials Research (Volumes 466-467)
• Pages: 146-151
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.466-467.146
• Online since: February 2012
• Authors: Yuan Cui, Yan Hui Li, Qian Duan
• Keywords: Collagen Gel, Scaffold, Self-Assemble

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