Novel Approach to Fabricate an Amperometric Biosensor by One-Step Electrodeposition Hemoglobin and Au Nanoparticles-Chitosan Nanocomposite Film on Carbon Ionic Liquid Electrode

Ai Li Sun; Gai Rong Chen; Qian Hua Zhu

doi:10.4028/www.scientific.net/AMR.482-484.164

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Novel Approach to Fabricate an Amperometric Biosensor by One-Step Electrodeposition Hemoglobin and Au Nanoparticles-Chitosan Nanocomposite Film on Carbon Ionic Liquid Electrode
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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 482-484Novel Approach to Fabricate an Amperometric...

Novel Approach to Fabricate an Amperometric Biosensor by One-Step Electrodeposition Hemoglobin and Au Nanoparticles-Chitosan Nanocomposite Film on Carbon Ionic Liquid Electrode

Abstract:

By one-step co-electrodeposition Hemoglobin (Hb) and Au nanoparticles-chitosan (Au NPS-Chi) nanocomposite film on carbon ionic liquid electrode(CILE), the Hb immobilized with Au NPS-Chi nanocomposite film was stable and retained its functional activity. The results of cyclic voltammetrys showed a pair of well-defined and quasireversible redox peak of Hb with the formal potentials of −0.545V (vs.SCE) in 0.1 mol/L pH 7.0 PBS; a linearity range for determination of H2O2 is from 0.4 to 208.4μmol/L with a detection limit of 0.12 μmol/L (S/N=3). As a results, the one-step co-electrodeposition and the promising feature of nanocomposite could serve as a versatile platform for the fabrication of electrochemical biosensors.