Flow Field Optimization and Strategy of Construction Cell Microspheres

Article Preview

Abstract:

Objective: To optimize process parameters and determine system of culture and collection cells microspheres by Fluent software. Method: Hypothesises of boundaries were brought forward of preparation cell microspheres. Parameters, the mesh and relevant conditions of cells microspheres were determined.Result: Parameters of the optimal process were determined by the flow field analysis of cell microspheres. Moreover the strategy of culture and collection cell microspheres were completed.Conclusion: The results may be caused research a equipment of proliferation cell micropheres. This is probably a consequence of reduction bankroll of development equipment of culture and collection cell micropheres.

You might also be interested in these eBooks

Info:

Periodical:

Advanced Materials Research (Volumes 482-484)

Pages:

2261-2264

Citation:

Online since:

February 2012

Authors:

Export:

Price:

Permissions CCC:

Permissions PLS:

Сopyright:

© 2012 Trans Tech Publications Ltd. All Rights Reserved

Share:

Citation:

[1] R.L. Carrier, M. Papadaki, M. Rupnick, F.J. Schoen, N. Bursac, R. Langer, L.E. Freed, G. Vunjak-Novakovic, Cardiac tissue engineering: cell seeding, cultivation parameters and tissue construct characterization, Biotechnol. Bioeng. 1999;64:580-589.

DOI: 10.1002/(sici)1097-0290(19990905)64:5<580::aid-bit8>3.0.co;2-x

Google Scholar

[2] Cooper JA, Lu HH, Ko FK, Freeman JW, Laurencin CT, Fiber-based tissue engineered scaffold for ligament replacement: design considerations and in vitro evaluation, Biomaterials. 2005;26:1523-1532.

DOI: 10.1016/j.biomaterials.2004.05.014

Google Scholar

[3] Dusting J, Sheridan J, Hourigan K, A fluid dynamic approach to bioreactor design for cell and tissue culture, Biotechnol. Bioeng. 2006;94:1197-1208.

DOI: 10.1002/bit.20960

Google Scholar

[4] Adebiyi AA, Taslim ME, Crawford KD. The use of computational fluid dynamic models for the optimization of cell seeding processes. Biomaterials.2011;32(34):8753-8770.

DOI: 10.1016/j.biomaterials.2011.08.028

Google Scholar

[5] Keck M, Haluza D, Selig HF, Jahl M, Lumenta DB, Kamolz LP, Frey M. Adipose tissue engineering: three different approaches to seed preadipocytes on a collagen-elastin matrix. Ann Plast Surg. 2011;67(5):484-488.

DOI: 10.1097/sap.0b013e31822f9946

Google Scholar

[6] Couet F, Mantovani D. A New Bioreactor Adapts to Materials State and Builds a Growth Model for Vascular Tissue Engineering.Artif Organs. 2011;1525-1594.[Epub ahead of print]

DOI: 10.1111/j.1525-1594.2011.01388.x

Google Scholar

[7] Lei XH, Ning LN, Cao YJ, Liu S, Zhang SB, Qiu ZF, Hu HM, Zhang HS, Liu S, Duan EK. NASA-Approved Rotary Bioreactor Enhances Proliferation of Human Epidermal Stem Cells and Supports Formation of 3D Epidermis-Like Structure.PLoS One. 2011;6(11):1-8.

DOI: 10.1371/journal.pone.0026603

Google Scholar