Enhanced Direct Electrochemistry of Glucose Oxidase and Glucose Biosensing Based on TiO₂-Decorated Graphene Nanohybrids

Abstract:

Using TiO2-decorated graphene (TiO2-G) nanohybrids as the immobilized platform for redox protein, and selecting glucose oxidase (GOD) as model enzyme, the direct electrochemistry of GOD was investigated in this paper. By virtue of the synergetic effect between graphene and TiO2 nanoparticles, the direct electron transfer of GOD on the TiO2-G nanohybrids modified electrode was remarkably promoted with a rate constant of 3.24 s-1. Moreover, based on the decrease of the electrocatalytic response of the reduced form of GOD by dissolved oxygen, a glucose biosensor was developed, which showed a satisfactory analytical performance for glucose determination over an acceptable linear concentration range from 5×10−4 to 2×10−2 mol/L. All these results demonstrated the great significance of such TiO2-G nanohybrids for the immobilization of enzyme and other biomolecules.

• Periodical: Advanced Materials Research (Volume 496)
• Pages: 507-510
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.496.507
• Online since: March 2012
• Authors: Jun Wu, Qian Liu, Kun Wang, Jian Rong Cai
• Keywords: Direct Electrochemistry, Glucose Biosensor, Glucose Oxidase, TiO₂-Decorated Graphene Nanohybrids

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