Comparison and Analysis of Hydrogen Production Capacity of 8 Strains of Marine Green Algae

Abstract:

In this study, four strains of Chlorella genus and four strains of Tetraselmis genus were investigated to research their in vitro hydrogenase activities and hydrogen productions. C.sp.-3 showed the second highest in vitro hydrogenase activity with 297.48 nmol H₂/(μg Chla h) and the highest volume of H₂ production with 10.246 μl/L among all the strains. Although T. sp.-3 exhibited a much low H₂ production of 0.298 μl/L, its in vitro hydrogenase activity was the highest with 315.92 nmol H₂/(μg Chla h). During the continuous culture of five weeks, the hydrogen production of C. sp.-3 reached the peak at 3rd weeks with 12.46 μl H₂ per liter culture, and decreased subsequently. In contrast, that of T. sp.-3 increased slowly and gradually with the culturing time, and was much lower than that of C. sp.-3 at each culture phases. These results showed that hydrogen production was a complex process that was determined not only by strain types but also by other factors, and that both C.sp.-3 and T. sp.-3 in the 8 strains were the most promising ones in hydrogen production and were worthy of further research.

• Periodical: Advanced Materials Research (Volumes 512-515)
• Pages: 1479-1483
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.512-515.1479
• Online since: May 2012
• Authors: Feng Ping Miao, Jing Cheng Zuo, Zheng Yi Liu, Song Qin
• Keywords: Hydrogen Production, Hydrogenase, Marine Green Algae

References

[1] A. Melis, L.P. Zhang, M. Forestier, M.L. Ghirardi and M. Seibert. Plant Physiol. 122 (2000) 127-135.

[2] S. Chader, H. Hacene and S.N. Agathos. Int. J. Hydrogen Energy. 34 (2009) 4941-4946.

[3] Y.F. Guan, M.C. Deng, X.J. Yu and W. Zhang. Biotechnol. Lett. 26(2004a) 1031-1035.

[4] Y.F. Guan, M.C. Deng, X.J. Yu and W. Zhang. Biochem. Eng J. 19 (2004b) 69-73.

[5] C.Q. Ran, X.J. Yu, M.F. Jin and W. Zhang. Biotechnol. Prog. 22 (2006) 438-443.

[6] F. Yan, Z.A. Chen, W. Li, X.P. Cao, S. Xue and W. Zhang. Process Biochem. 46 (2011) 1212-1215.

[7] M.L. He, L. Li and J.G. Liu. Int. J. Hydrogen Energy. 37 (2012) 4046-4056.

[8] C.F. Ji, J. Legrand, J. Pruvost, Z.A. Chen and W. Zhang. Int. J. Hydrogen Energy. 35 (2010), 7200-7205.

[9] T. Happe and J.D. Naber. Eur. J. Biochem. 214 (1993) 475-481.

[10] M. Winkler, A. Hemschemeier, C. Gotor, A. Melis and T. Happe. Int. J. Hydrogen Energy. 27(2002) 1431-1439.

[11] C.F. Ji, X. J.Yu, Z.A. Chen, S. Xue, J. Legrand and W. Zhang. Int. J. Hydrogen Energy. 36 (2011), 5817-5821.