Studies on Penicillium M2 Chitosanase Immobilized on CSG Hybrid Material by Cross-Linking Reaction

Yan Jun Wang; Shao Ling Zhuo; Sheng Chen

doi:10.4028/www.scientific.net/AMR.512-515.2419

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 512-515Studies on Penicillium M2 Chitosanase Immobilized...

Studies on Penicillium M2 Chitosanase Immobilized on CSG Hybrid Material by Cross-Linking Reaction

Abstract:

Chitosanase was immobilized on CSG hybrid material by cross-linking reaction and the immobilization conditions and characterization of the immobilized enzyme were carried out. The optimal conditions for immobilization were as follows: 0.1 g carrier material was treated with 2.5 ml 5% solution of glutaraldehyde and 2 ml chitosanase was immobilized on the carrier. Optimal temperature and pH for the immobilized enzyme were 60°C and 7.0, respectively. Kinetic parameter Km was10.64 g/l. The immobilized enzyme showed better operation and storage stability than the free enzyme.

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Periodical:

Advanced Materials Research (Volumes 512-515)

Pages:

2419-2423

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.512-515.2419

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Online since:

May 2012

Authors:

Yan Jun Wang, Shao Ling Zhuo, Sheng Chen

Keywords:

Chitosan, Chitosanase, CSG Hybrid Material, Immobilisation

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