Immobilized Methods and Application of Immobilized Saccharomyces cerevisiae Cells

Abstract:

The study was to find low costs and superior performance immobilized carrier for microorganism cells using in industry. Ca-alginate , Agar , Gelatin-glutaraldehyde and polyvinyl alcohol (PVA) were investigated to immobilize Saccharomyces cerevisiae cells, and immobilized cells’ production capacity for ethanol, contents of ethanol and mechanical strength were also studied. Then, optical microscope picture and scanning electronic microscope pictures were given to research its microenvironment. Finally, Batch and Continuous fermentation with PVA immbiolized Saccharomyces cerevisiae were experimented. The PVA immobilized method is the best in the four methods with concentration of 8%, microscopic observation also offered a proof that PVA material was fit for immobilizing cells with the traits of polycellular and reticulate. At last, the production capacity for ethanol and reactivity were superior to free cells in batch and continuous fermentation. This research could provide important information on the commercial utilization immobilized-cells for biotransformation.

• Periodical: Advanced Materials Research (Volumes 518-523)
• Pages: 218-222
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.518-523.218
• Online since: May 2012
• Authors: Ying Zhu, Jian Ping Zhou, Zhi Ye Wang
• Keywords: Immobilized Methods, Microscopic Observation, Production Capacity for Ethanol, PVA, Saccharomyces cerevisiae

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