Study on Specificity and Sensitivity of Oligonucleotide Probes for Fluorescence In Situ Hybridization

Xiao Dan Wang; Jiang Zhu; Takashi Someya

doi:10.4028/www.scientific.net/AMR.518-523.628

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Study on Specificity and Sensitivity of Oligonucleotide Probes for Fluorescence In Situ Hybridization
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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 518-523Study on Specificity and Sensitivity of...

Study on Specificity and Sensitivity of Oligonucleotide Probes for Fluorescence In Situ Hybridization

Abstract:

Sixteen bacterial strains were used to analyze the specificity and sensitivity of the commonly used oligonucleotide probe for FISH. The results showed that GAM42a probe was not only hybridized with γ-subclass proteobacteria strains, but also hybridized with other subclass proteobacteria strains. The specificity of GAM42a probe therefore would be improved. On the other hand, the average value of detection rates of ALF1b, HGCGP and LGCGP354B probe was less than 50%. After 10min lysozyme treatment, only the detection rate of LGCGP354B probe was improved.