Alkalibacillus huanghaiensis Sp. Nov., a New Strain of Moderately Halophilic Bacterias Isolated from Sea Water of the Yellow Sea in China

Abstract:

A Gram-positive, strictly aerobic and moderately halophilic bacterial strain HSD was obtained from the sea water sample collected from International Sea Water Bathing Place in Weihai, a city on the shore of the Yellow Sea. Strain HSD was bacillus. The optimum growth temperature was 32~37°C and the optimum pH was 6.0~9.0. It could grow well at 100 g•L-1 salt mass concentration. The G+C mole fraction of its genomic DNA was 37.5%. 16S rRNA gene sequence indicated a close relationship to Alkalibacillus halophilus (DQ359731) with similarity of 99%. However, they had obvious difference in whole-cell main fatty acid components, cell size, cell morphology, motility, oxidase, gelatine liquefication, nitrate reduction, NaCl tolerance range, pH tolerance range, G+C mole fraction, sole carbon source, sole nitrogen source, antibiotic sensitivity and a source of the strain isolation. Comparing of these taxonomic characters of strain HSD with those of other type strains of the genus Alkalibacillus, the differences were more obvious. In view of multiple identification results, it was proposed that strain HSD was classified as the type strain of a new species of the genus Alkalibacillus under the name Alkalibacillus huanghaiensis sp.nov..

• Periodical: Advanced Materials Research (Volumes 518-523)
• Pages: 8-15
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.518-523.8
• Online since: May 2012
• Authors: Wei Guo Li, Song Wen Tan, Zhong Xu, Hui Ru Yang, Li Wei, Jun Feng Su, Fang Ma
• Keywords: Alkalibacillus huanghaiensis sp. nov., Phylogeny, Taxonomy

References

[1] C.O. Jeon, J.M. Lim, J.M. Lee, Reclassification of Bacillus haloalkaliphilus Fritze 1996 as Alkalibacillus haloalkaliphilus gen nov, comb nov and the description of Alkalibacillus. salilacus sp nov, a novelhalophilic bacterium isolated from a salt lake in China, Int. J. Syst. Evol. Microhio1. 55 (2005) 1891–1896.

DOI: 10.1099/ijs.0.63456-0

[2] I. Romano, L. Lama, B. Nicolaus, A. Gambacorta and A. Giordano, Alkalibacillus filiformis sp. nov., isolated from a mineral pool in Campania, Italy, Int. J. Syst. Evol. Microbiol. 55 (2005) 2395–2399.

DOI: 10.1099/ijs.0.63864-0

[3] X.P. Tian, Y.Q. Zhang, S.K. Tang, L.H. Xu and C. L. Jiang, Isolation and identification one Alkalibacillus strain, Journal of Yunnan University (Natural Scionce Edition) 28(6) (2006) 539–543 (In Chinese).

[4] R. Usami, A. Echigo, T. Fukushima, T. Mizuki, Y. Yoshida and M. Kamekura, Alkalibacillus silvisoli sp. nov., an alkaliphilic moderate halophile isolated from non-saline forest soil in Japan, Int. J. Syst. Evol. Microhio1. 57 (2007) 770–774.

DOI: 10.1099/ijs.0.64713-0

[5] D. Fritze, Bacillus haloalkaliphilus sp nov., Int. J. Syst. Bacteriol. 46 (1996) 98–101.

[6] X.Z. Dong, M.Y. Cai, Common bacterium system appraisal handbook, Science Publishing Company, Beijing, 2001. (In Chinese)

[7] G.J. Holt, R.N. Krieg, H.P. Sneath, Bergey's ManUal of Determinative Bacteriology, 9th ed. Illiams & Wilkins Company, Baltimore, 1994.

[8] X. Xu, H.M. Wu, Y. Cao, Y.H. Wu and T. Zhang, Isolation and phylogenetic analysis of halophilic archaea AJ6, J. of Zhejiang University (Science Edition) 32(1) (2005) 83–88. (In Chinese)

[9] I. Romano, L. Lama, B. Nicolaus, A. Poli, A. Gambacorta and A. Giordano, Halomonas alkaliphila sp. nov., a novel halotolerant alkaliphilic bacterium isolated from a salt pool in Campania (Italy), J. Gen. Appl. Microbiol. 52(6) (2006) 339–48.

DOI: 10.2323/jgam.52.339

[10] H.N. Ross, M.D. Collins, B.J. Tindall, A rapid procedure for the detection of archae bacterial lipids in halopilic bacterial, J. Gen. Microl. 123(1) (1981) 75–80.

[11] T. Hasegawa, M. Takizawa, A.S. Tanid, A rapid analysis for chemical grouping of aerobic actinomycetes, J. Gen. Appl. Microbiol. 29 (1983) 319–322.

DOI: 10.2323/jgam.29.319

[12] H. Yi, J. Chun, Hongiella mannitolivorans gen. nov., sp. nov.,Hongiella halophila sp. nov. and Hongiella ornithinivorans sp. nov., isolated from tidal flat sediment, International Journal of Systematic and Evolutionary Microbiology 54 (2004) 157–162.

DOI: 10.1099/ijs.0.02861-0

[13] S.B. Kim, C.N. Seong, S.J. Jeon, K.S. Bae and M. Goodfellow, Taxonomic study of neutrotolerant acidophilic actinomycetes isolated from soil and description of Streptomyces yeochonensis sp. nov., International Journal of Systematic and Evolutionary Microbiology 54 (2004) 211–214.

DOI: 10.1099/ijs.0.02519-0

[14] N. Saitou, M. Nei, The neighbor joining method: a new method for reconstructing phylogenetic trees, J. Mol. Biol. Evol. 4(4) (1987) 406–425.

[15] J. Marmur, A procedure for the isolation of deoxyribonucleic acid from microorganisms, J. M. of Biol. 3 (1961) 208– 218.

[16] J. Marmur, P. Doty, Deterimination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature, J. Mol. Biol. 5 (1962) 109–118.

DOI: 10.1016/s0022-2836(62)80066-7

[17] D.J. Kushner, Life in high salt and solute concentrations: halophilic bacteria. Microbial Life in Extreme Environments, Academic Press, London, 1978, p.317– 368.