A Method for the Introduction of Acetylthiol Groups into Recombinant Human-Like Collagen

Yong Hui Wang; Chen Hui Zhu; Dai Di Fan; Yu Mi; Yan Sun

doi:10.4028/www.scientific.net/AMR.535-537.2416

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 535-537A Method for the Introduction of Acetylthiol...

A Method for the Introduction of Acetylthiol Groups into Recombinant Human-Like Collagen

Abstract:

Protected mercaptan groups may be introduced into Human-like collagen (HLC) under very mild conditions with S-acetylmercaptosuccinic anhydride(S-AMSA) as reagent. Fluorometric assay, Fluorescence Spectra and SDS-PAGE were employed to investigate the interaction between HLC and S-AMSA. These results obtained from this study provide theoretical evidence for the further investigate.

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Periodical:

Advanced Materials Research (Volumes 535-537)

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2416-2419

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.535-537.2416

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Online since:

June 2012

Authors:

Yong Hui Wang, Chen Hui Zhu, Dai Di Fan, Yu Mi, Yan Sun

Keywords:

Fluorometric Assay, Human-Like Collagen, S-Acetylmercaptosuccinic Anhydride

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