Validation of Fermentative Parameters for Efficient Succinate Production in Batch Operation by Actinobacillus succinogenes 130ZT

Abstract:

Succinate is an important platform molecule in the synthesis of a number of commodity and specialty chemicals. In the present study, the effects of different carbon and nitrogen sources, initial pH of the growth medium (pH 4.5-9.0), and temperature (25-45°C) on the fermentative succinate production by Actinobacillus succinogenes 130ZT were investigated in 100 mL anaerobic bottles. The results revealed that the highest concentration of succinate at 6.28 g/L was produced from 10 g/L of glucose or lactose in the medium containing 5 g/L yeast extract at 24 h. However, a comparable concentration of succinate was also produced when the medium was supplemented with 5 g/L spent brewer’s yeast extract. Based on these results, the cost effectiveness of succinate production could be improved by the use of glucose or lactose fermentation supplemented with spent brewer’s yeast extract. Optimized initial pH at 8.0, temperature at 37 °C, and inoculum size at 6% (v/v) provided the best succinate production at the concentration of 6.37 g/L with a yield of 68.73%.

• Periodical: Advanced Materials Research (Volumes 550-553)
• Pages: 1448-1454
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.550-553.1448
• Online since: July 2012
• Authors: Apichai Sawisit*, Supaluk Seesan, Sitha Chan, Sunthorn Kanchanatawee, Sirima Suvarnakuta Jantama, Kaemwich Jantama
• Keywords: Actinobacillus succinogenes, Batch Operation, Succinate

References

[1] D. Wang, Q. Li, Y. Mao, J. Xing and Z. Su, High-level succinic acid production and yield by lactose-induced expression of phosphoenolpyruvate carboxylase in ptsG mutant Escherichia coli, Appl Microbiol Biotechnol. 87 (2010) 2025-2035

DOI: 10.1007/s00253-010-2689-x

[2] J. McKinlay, C. Vieille and J. Zeikus, Prospects for a bio-based succinate industry, Appl Microbiol Biotechnol. 76 (2007) 727-740

DOI: 10.1007/s00253-007-1057-y

[3] M.V. Guettler, D. Rumler and M.K. Jain, Actinobacillus succinogenes sp. nov., a novel succinic-acid-producing strain from the bovine rumen, Inter J Syst Bacteriol. 49 (1999) 207-216

DOI: 10.1099/00207713-49-1-207

[4] H. Tanguler and H. Erten, Utilisation of spent brewer's yeast for yeast extract production by autolysis: The effect of temperature, Food Bioproducts Process. 86 (2008) 317-321

DOI: 10.1016/j.fbp.2007.10.015

[5] C. Andersson, D. Hodge, K.A. Berglund and U. Rova, Effect of Different Carbon Sources on the Production of Succinic Acid Using Metabolically Engineered Escherichia coli, Biotechnol Prog. 23 (2007) 381-388

DOI: 10.1021/bp060301y

[6] K.Q. Chen, J. Li, J.F. Ma, M. Jiang, P. Wei, Z.M. Liu and H.J. Ying, Succinic acid production by Actinobacillus succinogenes using hydrolysates of spent yeast cells and corn fiber, Bioresource Technol. 102 (2010) 1704-1708

DOI: 10.1016/j.biortech.2010.08.011

[7] P.C. Lee, W.G. Lee, S.Y. Lee, and H.N Chang, Effects of medium components on the growth of An. succiniciproducens and succinic acid production, Process Biochem. 35 (1999) 49-55

DOI: 10.1016/s0032-9592(99)00031-x

[8] L. Agarwal, J. Isar, G.K. Meghwanshi and R.K. Saxena, Influence of environmental and nutritional factors on succinic acid production and enzymes of reverse tricarboxylic acid cycle from Enterococcus flavescens, Enzyme Microbial Technol. 40 (2007) 629-636

DOI: 10.1016/j.enzmictec.2006.05.019

[9] M. J. Van der Werf, M.V. Guettler, M.K. Jain, J.G. Zeikus, Environmental and physiological factors affecting the succinate product ratio during carbohydrate fermentation by Actinobacillus sp. 130Z, Arch Microbiol. 167 (1997) 332-342

DOI: 10.1007/s002030050452

[10] L. Agarwal, J. Isar, G.K. Meghwanshi and R.K. Saxena, A cost effective fermentative production of succinic acid from cane molasses and corn steep liquor by Escherichia coli, J Appl Microbiol. 100 (2006) 1348-1354

DOI: 10.1111/j.1365-2672.2006.02894.x

[11] P.C. Lee, W.G. Lee, S. Kwon, S.Y. Lee and H.N. Chang, Succinic acid production by Anaerobiospirillum succiniciproducens: effects of the H2/CO2 supply and glucose concentration, Enzyme Microbial Technol. 24 (1999) 549-554

DOI: 10.1016/s0141-0229(98)00156-2