Relationship of Vitamin E Accumulation and Content of Sodium and Silicon in Cell Culture

Abstract:

The effect of Na and Si content on vitamin E accumulation in a wheat embryo callus culture was studied. Mathematical models were established to describe the growth kinetics and accumulation of vitamin E for the wheat embryo callus cells. With vitamin E accumulation as the objective, the best combination of Na and Si addition in the medium was selected through an optimum test. The results showed that the optimal production of vitamin E in Gamborg’s B5 medium was at the 0.80 mg/L NaH2PO4•H2O and 0.00%(w/w) H2SiO3. The degree of fit of the kinetics model of vitamin E accumulation was 99.11%, whereas the degree of fit of the kinetics model of cell growth was 98.68%, with a nonlinear relation. The beneficial elements Na and Si affected vitamin E accumulation in the wheat germ callus medium and the influence of Si was more significant than that of Na.

• Periodical: Advanced Materials Research (Volumes 550-553)
• Pages: 1529-1534
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.550-553.1529
• Online since: July 2012
• Authors: Heng Zhang, Zhao Tang Xu, Wen Qian Li, Hua Sun
• Keywords: Accumulation of Vitamin E, Nonlinear Model, Sodium and Silicon, Wheat Embryo

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