Optimization of the Conditions of Supercritical CO2 Extraction for Carotenoid from Blue Algae Using Response Surface Methodology

Da Hong Wang; Yan Fang Zhang; Teng Yin

doi:10.4028/www.scientific.net/AMR.550-553.1763

Paper Titles

Study on Separation and Purification of Resveratrol in Wine Grape Residue with Aqueous Two Phase Extraction Method
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Purification and Characterization of Limit Dextrinase from Malted Barley
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Thermal-Oxidative Aging Resistance and Water Absorption of Natural Rubber Latex/Methyl Methacrylate-Grafted-Natural Rubber Latex Blends
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Studies on Chemical Constituents of the Baphicacanthus cusia (Nees) Bremek Root
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Optimization of the Conditions of Supercritical CO₂ Extraction for Carotenoid from Blue Algae Using Response Surface Methodology
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Antioxidant Activities of Mirabilis jalapa L. Seed Epicarp Extract
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Preparaitve Separation and Purification of Cordycepin from Cultured Cordyceps militaris Using Cation Exchange Resin
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Comparison of Extracting Magnesium by Aluminothermic Reduction and Pidgeon Process
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Antioxidant Activities of Crude Extract and Chromatographic Fraction from Hsian-Tsao (Mesona Procumbens Hemsl.)
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Optimization of the Conditions of Supercritical CO₂ Extraction for Carotenoid from Blue Algae Using Response Surface Methodology

Abstract:

To optimize the conditions of supercritical CO2 extraction of carotenoid, a single factorial experiment was used to study the extracting temperature, time and pressure. A three-level Box–Behnken factorial design was used with response surface methodology (RSM) to optimize the three conditions. A mathematical model was then developed to show the effect of each factor and their interactions on the carotenoid yield. The single factorial experiment shows that the best extracting temperature, time and pressure are 50 0C,50 min and 25 MPa, respectively. The model estimates that a maximal caroteniod yield (0.624 mg/g) can be obtained when the conditions of temperature, time and pressure are set at 51.9 0C,61 min and 25.6MPa,repectively. The yield of carotenoid (0.621± 0.009 mg/g) from validation experiments agree with the predicted one.