T-RFLP Analysis of Soil Microbial Community from Shandong Province for Forensic Science

Abstract:

Soil microbial community very little had been found as trace evidence， however， it could be vital for forensic science. In this paper, soil microbial community diversities from seventeen different regions of Shandong province were investigated by Terminal Restriction Fragment Length Polymorphism (T-RFLP). The results of diversity index analysis show the highest Margalef index and Eveness index are 4.609 for sample D and 0.970 for sample E, respectively. In T-RFLP profile, the absolute advantage peak are 488bp (62.6% ) from sample O and 496.5bp (20.8%) from Sample H. The analysed terminal restriction fragments (T-RFs) are from Firmicutes and Proteobacteria. These results reveal that there are the obvious regional characteristic for microbial community of all the detected samples, which suggests T-RFLP analysis of microbial community diversity will be a rapid effective fingerprinting method for forensic detection.

• Periodical: Advanced Materials Research (Volume 599)
• Pages: 250-253
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.599.250
• Online since: November 2012
• Authors: Jia Jia Wang, Yu Mei Li, Zhi Wen Zhao, Qiang Li, Ying Zi Liu, Chun Jiang Ye, Da Ke Hao, Yan Hong Qu, Qing Qing Zhang, Jun Fa Li
• Keywords: Forensic Detection, Microbial Community Diversity, Soil, T-RFLP

References

[1] Liu W．T．, Marsh T．L．, Cheng H．, et a1. Characterization of microbial diversity by determining terminal restriction fragment length polymorphisms of genes encoding 16S rRNA． Appl. Environ. Microbiol, Vol. 63(1997), pp.4516-4522.

DOI: 10.1128/aem.63.11.4516-4522.1997

[2] Amann R.I, Ludwig W, Schleifer K.H. Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol Rev, Vol.59(1995), pp.143-169.

DOI: 10.1128/mr.59.1.143-169.1995

[3] Øvreås L, Torsvik V. Microbial diversity and community structure in two different agricultural soil communities. Microbiol Ecol, Vol. 36(1998), pp.303-315.

DOI: 10.1007/s002489900117

[4] Rossell-Mora R, Amann R. The species concept for prokaryotes. FEMS Microbiol Rev, Vol. 25(2001), pp.39-67.

[5] Hongning Song, et a1．Environmental impacts on bacterial communities diversity in sediments of Lake Dongping. Acta Microbiol Sin, Vol. 50(2010) , pp.1065-1071

[6] Lukow T，Dunfield P.F，Liesack W. Use of the T- RFLP technique to assess spatial and temporal changes in the bacterial community structure within an agricultural soil planted with transgenic and non- transgenic potato plants. FEMS Microbiol. Ecol, Vol. 32(2000), pp.241-247.

DOI: 10.1111/j.1574-6941.2000.tb00717.x