Mitomycin Induced Apoptosis in Human Leukemia K562 Cells

Abstract:

Objective: The research aimed to study the effects of mitomycin on human leukemic K562 cells, and to explore the mechanism of mitomycin induced apoptosis．In order to provide previous experiment basis for mitomycin applying clinical treatments Methods: The multiplication and apoptosis status of K562 cells treated different time by different concentration mitomycin were observed by light microscope, fluorescence microscope, TEM, agrose gel electrophoresis of DNA and flow cytometry. Results: The results showed that mitomycin could induce K562 cells apoptosis, and the best concentration was 12.5μg/ml for 48 h. The optimal concentration of apoptosis induced by apoptosis rate is （28.8±1.04）% （P<0.01）. Mitomycin could affect the S phase among cellular multiplication, cell could be blocked by mitomycin and then apoptosis in this phase. Conclusions: Mitomycin can induce the apoptosis of human leukemic K562 cells. It is of great significance to guide clinical medication.

• Periodical: Advanced Materials Research (Volume 599)
• Pages: 71-75
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.599.71
• Online since: November 2012
• Authors: Shu Li Shao, Bin Zhao, Wei Wei Zhang, Wei Zhao, Guang Hui Wu, Jin Ping Song
• Keywords: Apoptosis, K562 Cells, Mitomycin

References

[1] Ravandi V, Estrov Z1 Eradication of leukemia stem cells as a new goal of therapy in leukemia 1 Clin Cancer Res, Vol. 12 (2006), p.3401.

DOI: 10.1158/1078-0432.ccr-05-1879

[2] Zhao Q，Zhang H, Li Y, et a1．Anti-tumor effects of CIK combined with oxaliplatin in human oxaliplatin-resistant gastric cancer cells in vivo and in vitro．J Exp Clin Cancer Res,Vol.29 （2010）,pp.118-131．

DOI: 10.1186/1756-9966-29-118

[3] Fujiwara M, Muragaki Y, Ooshima A. Keloid-derived fibroblasts show increased secretion of factors involved in collagen turnover and depend on matrix metalloproteinase for migration. Br J Dermatol. Vol.153(2005),pp.295-300.

DOI: 10.1111/j.1365-2133.2005.06698.x

[4] Singh G,Sinha N.Involvement of apoptosis in mediating mitomycin C-induced teratogenesis in vitro.[J].Toxicology mechanisms and methods,Vol.20(2010),pp.190-196.

DOI: 10.3109/15376511003667859

[5] Krause S，Forster Y，Kraemer K，et a1．Vascular endothelial growth factor antisense pretreatment of bladder cancer cells significantly enhanccs the cytotoxicity of mitomycin C, genmcitabine and Cisvlatin[J]．J Urol，Vol.174(2005),pp.328-331．

DOI: 10.1097/01.ju.0000161588.94827.27

[6] Shan Miao,Xiao peng Shi,Hai Zhang, et al.Proliferation-Attenuating and Apoptosis-Inducing Effects of Tryptanthrin on Human Chronic Myeloid Leukemia K562 Cell Line in Vitro[J].International Journal of Molecular Sciences,Vol.12(2011),pp.243-245.

DOI: 10.3390/ijms12063831