Preparation and Characterization of Immobilized α-Amylase

Abstract:

P(DMAA-co-AM)hydrogel; adsorption method; a-amylase; Immobilized enzyme. Abstract. P(DMAA-co-AM)hydrogel was prepared by aqueous solution polymerization with N,N-dimethylacrylamide(DMAA) and acrylamide(AM) as monomers.,taking the P(DMAA-co-AM) hydrogel as carrier, a-amylase was immobilized by adsorption method.The investigation of effection of temperature and pH on enzymatic activity exhibites that the optimum temperatures are 50°C for both free and immobilized enzyme,the maximum activity is observed at pH 6.0 for both free and immobized enzyme. The activity of immobilized a-amylase remaines higher 40% at sixth run. Kinetic studies demonstrate that: Km values are 5.15749 and 1.44479 mg/mL for free and immobilized enzyme, Vmax values are 2.56082 and 0.58272 mg/mL min-1 for free and immobilized enzyme.

• Periodical: Advanced Materials Research (Volumes 602-604)
• Pages: 1186-1189
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.602-604.1186
• Online since: December 2012
• Authors: Zhi Jie Geng, Song Bai Lin, Gao Shuang Huang, Ai Ru Ke
• Keywords: A-Amylase, Adsorption Method, Immobilized Enzyme, P(DMAA-co-AM)Hydrogel

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