Fuel Ethanol Production from Lignocellulosic Biomass Using a Recombinant Yeast Strain

Ming Chen; Guo Ren Zu; Chun Zhi Zhang

doi:10.4028/www.scientific.net/AMR.608-609.281

Paper Titles

Application Status and Development Strategies of Biomass Energy in China
p.261

Overview of Methods to Remove Solid Particles from Biomass Fast Pyrolysis Oils
p.265

Influence of Micro-Emulsified Biodiesel on Combustion and Emission Characteristics of a Turbocharged Diesel Engine
p.269

Study on the Emission Properties of Biodiesel Fueled on Medium-Sized Diesel Engine under Steady Working Conditions
p.275

Fuel Ethanol Production from Lignocellulosic Biomass Using a Recombinant Yeast Strain
p.281

Rational Use of Residential Digesters for Sewage Treatment with Carbon Credits
p.286

Optimization of Dilute Acid Hydrolysis of Corn Stover for Separate Production of Xylose and Glucose by Response Surface Methodology
p.298

Hot Utilization of Fuel Gas in Fluidized Bed Biomass Gasification
p.302

The Research on Influence of Intensive Straw-Gasifying on Household Energy Consumption in the Countryside
p.308

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Fuel Ethanol Production from Lignocellulosic Biomass Using a Recombinant Yeast Strain

Abstract:

Fuel ethanol production from lignocellulosic biomass corn stover was investigated. Compared with acid pretreatment and ammonia steeping pretreatment, alkali pretreatment with 2% NaOH markedly enhanced lignin removal and thereby improved the enzymatic hydrolysis yield to 81.2% by 48 h. Fed-batch hydrolysis was started with a batch hydrolysis containing 80 g/l substrate, with cellulosic residue added at 6 and 12 h twice to get a final substrate concentration of 110 g/l. After 72 h of hydrolysis, the reducing sugar concentration reached 89.5 g/l with a hydrolysis yield of 83.3%. Further fermentation of the cellulosic hydrolysate containing 56.7 g/l glucose and 23.6 g/l xylose was performed using a recombinant yeast strain Saccharomyces cerevisiae ZU-10, and 36.3 g/l ethanol was produced within 72 h. The research results are meaningful in fuel ethanol production from renewable lignocellulosic biomass.