Rapid and Sensitive Competitive Fluorescence Immunoassay for the Detection of Chlortetracycline Residues in Environment

Abstract:

An direct competitive immunofluorescence method was developed in plate to detect chlortetracycline residues in environment using the Lumichrome as fluorescent probe. Different reaction conditions such as pH , the concentration of antigen and antibody and incubation times were assayed. Under optimized conditions, chlortetracycline can be determined in the concentration range of 1×10-8--2×10-4 mol/L, and the linear equation was y=11.363x+93.026, R2=0.99. The detection limit of this method IC90 was 3.74×10-7mol/L. The recoveries of chlortetracycline at various spiking levels in the sludge samples ranged from 95.43% to 104.26%, with relative standard deviations of 3% to 6%. These data demonstrate that the excellent performance of the proposed method in the detection of chlortetracycline in water samples.

• Periodical: Advanced Materials Research (Volumes 610-613)
• Pages: 1166-1169
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.610-613.1166
• Online since: December 2012
• Authors: Ying Xian Yuan, Zhao Hui Li, Gui Zhi Jiao
• Keywords: Chlortetracycline, Fluorescence Probe, Immunofluorescence, Lumichrome

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