The Effect of PHA on Enhanced Biological Phosphorus Removal

Abstract:

Used A/O-SBR(anaerobic/aerobic alternating enhanced biological phosphorus removal system) to study carbon source and phosphate accumulating organisms (PAO) intracellular energy storage substance transformation, also studied PAO’s phosphorus removal capacity. PAO could uptake quick degradation organic matters and synthesis polyhydroxyalkanoates(PHA). PHA was a kind of intracellular energy storage substance, its content could affect PAO’s phosphorus removal capacity. When carbon source was plentiful, PAO could synthesis a lot of PHA, phosphorus removal capacity could reach 38.16 mgp/gvss. Sludge retention time could affect PAO synthesis PHA of quantity and structure. Along with the sludge retention time growth, intracellular PHA content was increased, and phosphorus uptake of desired aerobic time continues to decrease, so increasing the phosphorus removal efficiency. When the municipal sewage as carbon source, PHA was mainly composed of PHB(poly-ß-hydroxyvalerate) and PHV(poly-ß-hydroxyvalerate). Along with the sludge retention time prolongation, PAO could synthesis more PHV, PHB content was remain unchanged, PHA composition structure was changed.

• Periodical: Advanced Materials Research (Volumes 610-613)
• Pages: 1343-1348
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.610-613.1343
• Online since: December 2012
• Authors: Wei Han*, Linjiang Yuan, Lu Chai, Kai Zhao, Guang Zhu Li
• Keywords: Enhanced Biological Phosphorus Removal (EBPR), PHA, PHB, PHV, Sludge Retention Time (SRT)

References

[1] T. Mion,V. Arun, Y. Tsuzuki, T. Matsuo, Effect of phosphorus accumulation on acetate metabolism in the biological phosphorus removal,in: R. Ramadori(Ed.),Biological Phosphorus Removal From Wastewaters (Advances in Water Pollution Control 4), Pergamon Press,Oxford,1987,pp.27-38.

DOI: 10.1016/b978-0-08-035592-4.50009-2

[2] V. Arun, T. Mino, T. Matsuo, Biological mechanism of acetate uptake mediated by carbohydrate consumption in excess phosphorus removal systems, Water Res. 22(1988)565-570.

DOI: 10.1016/0043-1354(88)90056-5

[3] H. Satoh, T. Mino, T. Matsuo, Uptake of organic substrates and accumulation of polyhydroxyalkanoates linked with glycolysis of intracellular carbohydrates under anaerobic conditions in the biological excess phosphate removal processes, Water Sci. Technol. 26(5-6)(1992)933-942.

DOI: 10.2166/wst.1992.0535

[4] G.J.F. Smolders, J. Van der Meij, M.C.M. van Loosdrecht, J.J. Heijnen, Model of the anaerobic metabolism of the biological phosphorus removal process: stoichiometry and ph influence, Biotechnol. Bioeng. 42(1994)461-470.

DOI: 10.1002/bit.260430605

[5] G.J.F. Smolders, J. Van der Meij, M.C.M. van Loosdrecht, J.J. Heijnen, Stoichiometric model of the aerobic metabolism of the biological phosphorus removal process, Biotechnol. Bioeng. 44(1994)837-848.

DOI: 10.1002/bit.260440709

[6] H. Pereira, P.C. Lemos, M.A.M. Reis, J.P.S.G. Crespo, M.J.T. Carrondo, H. Santos, Model for carbon metabolism in biological phosphorus removal processes based on in vivo 13 C-NMR, Water Res. 30(1996)2128-2138.

DOI: 10.1016/0043-1354(96)00035-8

[7] ZHENG Xingcan, LI Yaxin. Phosphorus and nitrogen removal from wastewater technology[M]. Beijing: China Building Industry Press,1998.

[8] S.H. Chuang, C.F. Ouyang, H.C. Yuang, et al. Effects of SRT and DO on nutrient removal in a combined AS-biofilm process[J]. Water Science and Technology,1997,36(12):19-27.

DOI: 10.2166/wst.1997.0426

[9] LIU Yan, CHEN Yinguang, ZHENG Hong, et al. Effect of different ratios of propionic to acetic acid on phosphorus removal by an enriched culture of phosphorus accumulating organisms[J]. Acta Scientiae Circumstantiae(In Chinese),2006,26(8):1278-1283.

[10] Andrew Amis Randall, Yan-Hua Liu. Polyhydroxyalkanoares from potentially a key aspect of aerobic phosphorus uptake in enhanced biological phosphorus removal[J]. Water Research,2002,36(1):3473-3478.

DOI: 10.1016/s0043-1354(02)00047-7

[11] Ministry of Environmental Protection of the People's Republic of China, Water and wastewater monitoring analysis method(Fourth Edition)[M]. Beijing: China Environmental Science Press,2002.

[12] YVES COMEAU, KENNE TH J. HALL, AND WILLIAM K. Determination of Poly-β-Hydroxybutyrate and Poly-β-Hydroxyvalerate in Activated Sludge by Gas-Liquid Chromatography. OLDHAM APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Sept.1988, P.2325-2327vol,54,No.9

DOI: 10.1128/aem.54.9.2325-2327.1988

[13] Pijuan M, Saunders A M, Guisasola A, et al. Enhanced biological phosphorus using propionate as the sole carbon source [J] . Biotech Bioeng, 2004, 85(1): 56-67.

DOI: 10.1002/bit.10813

[14] Oehmen A, Zeng R J, Yuan Z, et al. Anaerobic metabolism ofpropionate by polyphosphate-accumulating organisms in enhanced biological phosphorus removal systems[J]. Biotech Bioeng, 2005,91(1): 43-53.

DOI: 10.1002/bit.20480

[15] Lu H, Oehmen A, Virdis B, et al. Obtaining highly enrichedcultures of Candidatus Accumulibacter phosphates through alternating carbon sources [J]. Water Research, 2006, 40(20): 3838-3848.

DOI: 10.1016/j.watres.2006.09.004

[16] ZHANG Chao, CHEN Yinguang, LIU Yan.Effect of different ratios of propionic to acetic acids on long-term cultured active sludge for enhanced biological phosphorus removal[J]. Environmental Science(In Chinese),2008,29(9):2548-2552.

[17] Wang N D, Peng J, Hill G. Biological model of glucose induced enhanced biological phosphorus removal under anaerobic condition[J].Water Research,2002, 36(1):49~58.

DOI: 10.1016/s0043-1354(01)00236-6