Construction of Recombinant E.coli Expressing a Thermophilic Catalase and its Application in H₂O₂ Wastewater Treatment

Abstract:

The recombinant plasmid pETac-CATHis with tac promoter was constructed for the constitutive expression of a thermophilic catalase in E.coli. And the effects of cultivation conditions of two recombinant strains, BL21(DE3)/pETac-CATHis and BL21(DE3)pLysS/pETac-CATHis, such as initial pH, cultivation temperature, cultivation time, and loading volume on catalase activity were investigated. The optimal cultivation conditions were: the initial pH 7.0, cultivation temperature 37°C, cultivation time 12 hours, and loading volume 25mL in 100mL flask. Under the optimal cultivation condition, the highest catalase activities of the two recombinant strains were 291.7 U/mL and 349.5 U/mL, respectively. After adding catalase, H₂O₂ in simulation H₂O₂ wastewater containing phenol was decomposed completely very quickly. Declining of catalase activity was detected with enhancing the phenol concentration, probably because the phenol might be a kind of competitive inhibitor for catalase.

• Periodical: Advanced Materials Research (Volumes 610-613)
• Pages: 1588-1594
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.610-613.1588
• Online since: December 2012
• Authors: Yan Hong Chang*, Chun Hong Shi, Yao Zhou, Hui Luo
• Keywords: Catalase, Cultivation Conditions, H₂O₂ Wastewater, Recombinant Strains

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