Construction of Recombinant E.coli Expressing a Thermophilic Catalase and its Application in H2O2 Wastewater Treatment

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Abstract:

The recombinant plasmid pETac-CATHis with tac promoter was constructed for the constitutive expression of a thermophilic catalase in E.coli. And the effects of cultivation conditions of two recombinant strains, BL21(DE3)/pETac-CATHis and BL21(DE3)pLysS/pETac-CATHis, such as initial pH, cultivation temperature, cultivation time, and loading volume on catalase activity were investigated. The optimal cultivation conditions were: the initial pH 7.0, cultivation temperature 37°C, cultivation time 12 hours, and loading volume 25mL in 100mL flask. Under the optimal cultivation condition, the highest catalase activities of the two recombinant strains were 291.7 U/mL and 349.5 U/mL, respectively. After adding catalase, H2O2 in simulation H2O2 wastewater containing phenol was decomposed completely very quickly. Declining of catalase activity was detected with enhancing the phenol concentration, probably because the phenol might be a kind of competitive inhibitor for catalase.

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Advanced Materials Research (Volumes 610-613)

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1588-1594

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December 2012

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© 2013 Trans Tech Publications Ltd. All Rights Reserved

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