Comet Assay Determine DNA Damage Baseline Level of Mollusca Hemolymph Cells in Poyang Lake and Surrounding Waters

Abstract:

Mollusc species were sampled in different sites based on pollution level in Poyang lake and its surrounding waters during withered water period (from Oct to Dec) and plentiful water period (from May to June) from 2007 to 2008. Single-cell gel electrophoresis (SCGE) was used to analyze their haemolymph cells Arbitrary Unit(AU) and Olive tailmoment (OTM) value of DNA damage in molluca. The results showed that the mean DNA damage value Jin river>Nanxin>Xiu stream in withered water period; and in Plentiful water period, Hukou>Qinglan lake>Xiyuan. Two mollusc species Corbicula fluminea and Bellamya aeruginosa have higher DNA damage value than other mussel in all sites. Comparing the DNA damage value of Bellamya aeruginosa collected in twenty sites in 2008, the highest DNA damage value was observed in Dacha lake and lowest in Xiyuan. Meanwhile, the highest DNA damage value of Unio douglasiae was recorded Chucha river from ten sites, and lowest in Xiyuan river.

• Periodical: Advanced Materials Research (Volumes 610-613)
• Pages: 952-960
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.610-613.952
• Online since: December 2012
• Authors: Liang Xu, Kan Zhou, Chun Hua Zhou, Shan Ouyang, Xiao Ping Wu
• Keywords: Comet Assay, DNA Damage, Mollusc Haemolymph Cell, Poyang Lake

References

[1] Y. Tian, X.Y. Lu, K. Yi, X.J. He, C.J. Huang, L. Xu (2004). Chem. Life, 24 (1): 77-78.

[2] I.Y. Kim, C.K. Hyun (2006). Ecotoxicol. Environ. Saf., (64): 288-297.

[3] Y.Y. Liu, W.Z. Zhang, Y.X. Wang, E.Y. Wang (1979). Chinese economic zoography-Fresh mollusk. In Chinese.

[4] G. Juhel, J. O'Halloran, S.C. Culloty, R.M. O'Riordan, J. Davenport, N.M. O'Brien, K.F. James, A. Furey, O. Allis (2007). Environ. Mol. Mutagen., 48: 22-29.

[5] J.B. Culbertson, I. Valiela, Y.S. Olsen, C.M. Reddy (2008). Environ. Pollut., 154: 312-319.

[6] L. Peperzak, M. Poelman (2008). J. Sea Rese., 60 (3): 220-222.

[7] V. Besada, J.M. Andrade, F. Schultze, J. Fumega, B. Cambeiro, J.J. González (2008). Journal of Marine Systems, 72: 320-331.

DOI: 10.1016/j.jmarsys.2007.03.012

[8] C. Riva, A. Binelli, A. Provini (2008). Environ. Pollut., 151: 652-662.

[9] S. Einsporn, A. Koehler (2008). Mar. Environ. Res., 66: 185-186.

[10] R. Bocchetti, C.V. Lamberti, B. Pisanelli, E.M. Razzetti, C. Maggi, B. Catalano, G. Sesta, G. Martuccio, M. Gabellini, F. Regoli (2008). Mar. Environ. Res., 66: 24-26.

DOI: 10.1016/j.marenvres.2008.02.013

[11] C. Bolognesi, A. Buschini, E. Branchi, P. Carboni, M. Furlini, A. Martino, M. Monteverde, P. Poli, C. Rossi (2004). Sci. Total Environ., 333: 127-136.

DOI: 10.1016/j.scitotenv.2004.05.018

[12] A. Sarkar, D.C.S. Gaitonde, A. Sarkar, D. Vashistha, C. D'Silva, S.G. Dalala (2008). Ecotoxicol. Environ. Saf., 71 (2): 473-482.

[13] N.P. Singh, M.T. Mccoy, R.R. Tice (1988). Exp Cell Res, 175 (2): 184-191.

[14] B.N. Qi, G.H. Tang, J.S. Li, J.H. Yi, J.F. Guo, J.L. Miao (2006). Chinese J Ind Med June, 19, 3: 163-165.

[15] L.Y. Xue, L. Li, J.S. Zhou (2005). Reservoir Fish., 25 (3): 8-9.

[16] Y.Q. Li, L.W. Sun, M.M. Qu, Z.M. Kong, Z.T. Liu (2004). Res.Environ.Sci., 17 (5): 35-38.

[17] Q. Liu, N.H. Jiang, J. Li, W.S. Tang , Z.Q. Wang, Y.C. Zhao, F.Y. Fan (2006). Chin J Radiol Med Prot, 26 (6): 573-575.

[18] Y. Qiao, Z.S. Lu, H.C. Yao, X. Yang, R. Li (2004). J Health Toxlcol, 18 (3): 190-193.

[19] W.H.L. Siu, J. Cao, R.W. Jack, R.S.S. Wu, B.J. Richardson, L. Xu, P.K.S. Lam (2004). Aquat. Toxicol., 66: 381-392.

[20] R.F. Lee, S. Steinert (2003). Mutat Res, 544: 43-64.

[21] Information on http://jiangxi.jxnews.com.cn/system/2008/04/24/002736012.shtml

[22] Information on http://www.jxsl.gov.cn/article.jsp?articleid=6706

[23] Information on http://blog.searchina.net.cn/guoruiqun163/blogview.asp?logID=3992

[24] Information on http://www.jxsl.gov.cn/article.jsp?articleid=8836

[25] Information on http://www.jxgdw.com/jxgd/news/jxzh/userobject1ai616469.html

[26] G. Frenzilli, M. Nigro, V. Scarcelli (2001). Aquat. Toxicol., 53: 19-32.

[27] I.V. Villela, I.M. Oliveira, J.C. Silveira, J.F. Dias, J.A.P. Henriques, J.D. Silva (2007). Mutat. Res., 628: 76-86.

[28] C. Pellacani, A. Buschini, M. Furlini, P. Poli, C. Rossi (2006). Aquat. Toxicol., 77: 1-10.

[29] I.V. Villela, I.M. Oliveira, J. Silva (2006). Mutat. Res., 605: 78-86.

[30] B. Laffon, T. Rabade, E. Pasaro, J. Mendez (2006). Environ. Int., 32: 342-348.

[31] Q. Zhao, L.J. Duan, Y.S. Liu (2004). J. Pub Health and PrevMed, 15 (5): 57-59.

[32] J. Yang, A.J. Shi (2002). Study on the Classification in Hemocytes of Three Kind of Freshwater Pearl Clams. J. Sichuan Univ. (Nat. Sci. Ed.), 4 (39): 68-72.

[33] Y.Q. Shao, Y. Li, M.L. Qing (2005). Periodical of ocean univ. China, 35 (3): 404-406.