Isolation and Characterization of Cellulose Producing Bacterial Strain from Orange Pulp

Neha Tyagi; Sumathi Suresh

doi:10.4028/www.scientific.net/AMR.626.475

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HomeAdvanced Materials ResearchAdvanced Materials Research Vol. 626Isolation and Characterization of Cellulose...

Isolation and Characterization of Cellulose Producing Bacterial Strain from Orange Pulp

Abstract:

Cellulose producing bacterial strain was isolated from orange pulp. The isolated strain was identified to be Gluconoacetobacter intermedius NT based on several morphological characteristics, biochemical tests and 16S r-DNA analysis. Bacterial cellulose production by the isolated bacterial strain was maximum in Hestrin and Schramm (HS) and modified HS media (8.5 and 10 g /L, respectively) following cultivation under static conditions for 10 days. Modified HS medium containing 40 ml/L of heat pre-treated molasses as the sole source of carbon facilitated significant production of cellulose (3.5 g/L) by the bacterial isolate. Utilization of molasses as the carbon source for the production of bacterial cellulose would have an advantage over pure sugars from the commercial point of view.

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Advanced Materials Research (Volume 626)

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475-479

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https://doi.org/10.4028/www.scientific.net/AMR.626.475

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Online since:

December 2012

Authors:

Neha Tyagi, Sumathi Suresh

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Bacterial Cellulose, Gluconoacetobacter intermedius, Molasses, Orange Pulp

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