Label-Free Aptamer Fluorescence Determination of Trace Pb²⁺ Using AuPd Nanoalloy Probe as Catalyst

Abstract:

In the condition of 1.24 mmol/L EDTANa₂, 16.7 mmol/L NaCl and 0.17 mmol/L Tris, the substrate chain of double-stranded DNA (dsDNA) could be cracked by Pb²⁺ to release single-stranded DNA (ssDNA) that adsorb onto AuPd nanoparticle (AuPdNP) and form stable AuPdNP-ssDNA, but the dsDNA can not protect AuPdNP that were aggregated to big AuPdNP aggregations (AuPdNPA) under the action of NaCl. The AuPdNP-ssDNA and AuPdNPA could be separated by centrifugation. With the concentration of Pb²⁺ increased, the released ssDNA increased, the AuPdNP-ssDNA in centrifugation solution increased and the catalytic effect enhanced on the fluorescence quenching reaction of Rhodamine 6G (Rh6G) and NaH₂PO₂, which led the fluorescence intensity at 552nm to decrease. The decreased fluorescence intensity (ΔF_552nm) was linear to the concentration of Pb²⁺ in the range of 0.33-8.00 nmol/L, a detection limit of 0.21 nmol/L. The proposed method was applied to detect Pb2+ in water samples, with satisfactory results.

• Periodical: Advanced Materials Research (Volumes 631-632)
• Pages: 18-21
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.631-632.18
• Online since: January 2013
• Authors: Zhi Liang Jiang, Mei Ling Tang, Qing Ye Liu, Ai Hui Liang
• Keywords: AuPd Nanoalloy, Fluorescence, Label-Free Aptamer, Nanocatalysis, Pb²⁺

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