Preparative Separation and Purification of Lancifodilactone C from Schisandra Chinensis (Turcz.) Baill by High-Speed Counter-Current Chromatography

Abstract:

High-speed counter-current chromatography (HSCCC) was successfully applied to the preparative separation and purification of lancifodilactone C from the crude extracts of Schisandra chinensis (Turcz.) Baill. Following an initial cleaning-up step on the AB-8 macroporous resin, a preparative high-speed counter-current chromatography (HSCCC) with a two-phase solvent system composed of Chloroform- n-Butyl alcohol- methanol -water(10:0.5:8:4，v/v) was used to isolate and separate lancifodilactone C from Schisandra Chinensis(Turcz.) Baill. A total of 101 mg lancifodilactone C with purities of 98.2% were obtained from 1000 mg crude extract in one-step elution and less than 1 h, and the structure identification was performed by UV, IR, MS, ¹H NMR and ¹³C NMR.

• Periodical: Advanced Materials Research (Volumes 634-638)
• Pages: 1502-1505
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.634-638.1502
• Online since: January 2013
• Authors: Bin Li, Xian Jun Meng, Li Jie Zhu, Xin Yao Jiao
• Keywords: Counter-Current Chromatography, Isolation, Preparative Chromatography, Schisandra Chinensis(Turcz.) Baill

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