Comparison between Dextran-Coated Superparamagnetic Iron Oxide Nanoparticles and Magnetoliposome on Rabbit Corneal Endothelial Cells Labeling

Abstract:

Purpose: To evaluate and compare the promising applications of dextran-coated superparamagnetic iron oxide nanoparticle (dSPION) and magnetoliposome on corneal endothelial cells transplant therapy. Methods: Two kinds of novel superparamagnetic iron oxide nanoparticles (SPIONs), dSPION and magnetoliposome were synthesized, characterized and incorporated to rabbit corneal endothelial cells (RCECs). Uptakes of SPIONs were identified by Prussian blue staining. Cells migrating rates in magnetic field were calculated. Cytotoxicities of the two SPIONs were detected using MTT assay. Result: Magrating rates of dSPION (4 ~ 64 μg/mL) labled RCECs were 24.1±2.4% ~ 78.3±2.2%, and 5.0±1.7% of unlabeled RCECs. (p0.05) Conclusion: RCECs labeled with each SPION migrated significantly in external magnetic field, while cytotoxicities did not revealed. Our study demonstrated the potential applications of dSPION and magnetoliposome on labeling RCECs for cell transplantation.

• Periodical: Advanced Materials Research (Volumes 652-654)
• Pages: 234-240
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.652-654.234
• Online since: January 2013
• Authors: Ming Feng Wu, Li Xia Lu, Xiao Ting Sun, Fei Du, Yan Long Bi
• Keywords: Corneal Endothelial Cells Cytotoxicity, Magnetoliposome, Superparamagnetic Iron Oxide Nanoparticles

References

317±0. 017.

323±0. 016.

328±0. 025.

323±0. 008.

321±0. 026 p value.

103.

215.

476.

143.

254 Table 2. Absorbance values of magnetoliposome magnetoliposome（μg/mL） 16 32 64 128 256 absorbance value.

319±0. 015.

328±0. 021.

320±0. 011.

331±0. 022.

328±0. 020 p value.

103.

464.

106.

603.

445 Discussion In prospect, cell transplantation will become a novel therapy for incurable diseases. [7, [] Orlic D, Kajstura J, Chimenti S, et al: Mobilized bone marrow cells repair the infarcted heart, improving function and survival. Proc Natl Acad Sci USA 98: 10344-10349, 2001. , [] Sato Y, Araki H, Kato J, et al: Human mesenchymal stem cells xenografted directly to rat liver are differentiated into human hepatocytes without fusion. Blood 106: 756-763, 2005. , [] Qian H, Yang H, Xu W, et al: Bone marrow mesenchymal stem cells ameliorate rat acute renal failure by differentiation into renal tubular epithelial-like cells. Int J Mol Med 22: 325-332, 2008 ] Several studies have demonstrated the feasibility of tansplanting cultured CECs as a solution for the shortage problem of donor corneas[7, [] Griffith, M., Osborne, R., Munger, R., Xiong, X., Doillon, C.J., Laycock, N.L., Hakim, M., Song, Y., Watsky, M.A., 1999. Functional human corneal equivalents constructed from cell lines. Science 286, 2169–2172. , [] Engelmann, K., Drexler, D., Bohnke, M., 1999. Transplantation of adult human or porcine corneal endothelial cells onto human recipients in vitro. Part I: Cell culturing and transplantation procedure. Cornea 18, 199–206. , [] Mimura T, Yamagami S, Usui T, et al. Long-term outcome of iron-endocytosing cultured corneal endothelial cell transplantation with magnetic attraction. Exp Eye Res 2005; 80(2): 149–157. ]. Mimura et al. previously used magnetic force to attract labled RCECs to rabbit recipient corneas for transplantation. [18] In consideration of the high hysteresis of iron power which might lead to cells self-aggregation, Patel et al. labeled human corneal endothelial cells with SPION and transplanted the cells in a human anterior segment perfusion organ culture model. [8] There was little detail about synthesis and surface-modified of the SPIONs in Patel's reported. In our study, two kinds of SPIONs were synthesized, characterized and incorporated to RCECs for evaluating the potential application of these nanoparticle on cells transplantation, offering more options and comparisons. dSPION used in our study was similar with commercial dextran coated SPIONs such as Feridex, Endorem, Combidex and Sinerem, which were approved for MRI in patients. [11, 12] In another report, liposomes incorporating magnetic nanoparticles(magnetoliposomes) were filled with drugs and used for targeted delivery applications. [] T. Kubo, T. Sugita, S. Shimose, Y. Nitta, Y. Ikuta, T. Murakami, Targeted systemic chemotherapy using magnetic liposomes with incorporated adriamycin for osteosarcoma inhamsters, International Journal ofOncology18 (1) (2001).