A Study of Strain Distribution and Cell Adhesion of MicroHA/CS Composite Scaffold under Mechanical Loading

Abstract:

Mechanical stimulus is one of the factors that affect cell proliferation and differentiation in the process of bone tissue regeneration. It is difficult to directly measure stress and strain inside the scaffold, so we decide to use FEA (finite element analysis) to obtain the strain result. A uniform displacement equivalent to an uniaxial strain of 2500、3000、3500、4000、4500、5000με was applied to the solid material phase. These results suggested that apparent strain of 3000με was best suit to the physiology environment, which including more inner strain elements between 1000 and 3000με than others. When the strain more than 3000με, the number of nodes of more than 4000 μ ε increased obviously. This will cause damage to cells which adhesion in support of the pore wall. In vitro, MC3T3-E1 preosteoblast cells were used to investigate cell attachment, spreading and proliferation on the scaffolds via SEM (scanning electron microscopy). The results showed that cells could adhesion and proliferation well after 7 and 14 days under the dynamic loading of 3000με.

• Periodical: Advanced Materials Research (Volume 668)
• Pages: 565-570
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.668.565
• Online since: March 2013
• Authors: Jian Ming Zhang, Rui Xin Li, Ting Ting Ren, Cai Hong Shi, Hao Li, Zong Ming Wan, Yong Guo, He Yan Wang, Xi Zheng Zhang
• Keywords: Finite Element, Mechanical Stimuli, Micro Computed Tomography, MicroHA/CS Composite Scaffold

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