Research on Improvement of Nitrogenase Activity of Free-Living Nitrogen-Fixing Bacteria Using DBD Plasma

Yan Yun Zhu; Xiao Li Zhu; Fang She Yang

doi:10.4028/www.scientific.net/AMR.671-674.2674

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 671-674Research on Improvement of Nitrogenase Activity of...

Research on Improvement of Nitrogenase Activity of Free-Living Nitrogen-Fixing Bacteria Using DBD Plasma

Abstract:

Nitrogen-fixing bacteria were screened from the rhizosphere soil of plants in Shaanxi in China. 36 free-living nitrogen-fixing bacterial strains were isolated and their nitrogenase activity were determined by acetylene reduction assay (ARA), two strains named FLNB03 and FLNB09 with higher nitrogenase activity were isolated and identified by 16S rRNA sequencing. The datum showed that FLNB03 was similar to Acinetobacter and their similarity reached 99%, FLNB09 was similar to Agrobacterium sp. and their similarity reached 99%. Then both of them were treated using Dielectric Barrier Discharge (DBD) plasma for mutation and their mutants called FLNB03-2 and FLNB09-3 were obtained. The nitrogenase activity of FLNB03-2 was 0.61±0.10 nmol•107cfu-1•h-1, and that of FLNB09-3 was 0.40±0.05 nmol•107cfu-1•h-1, their nitrogenase activity increased by 22.00% and 14.29% than their original bacteria respectively. FLNB03-2 and FLNB09-3 might be used as microbial fertilizer.

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Advanced Materials Research (Volumes 671-674)

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2674-2678

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.671-674.2674

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Online since:

March 2013

Authors:

Yan Yun Zhu, Xiao Li Zhu, Fang She Yang

Keywords:

Dielectric Barrier Discharge (DBD) Plasma, Free-Living Nitrogen-Fixing Bacteria, Nitrogenase Activity (NA)

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