Study on MR Imaging of Endothelial Progenitor Cells Labeled with the Complex of Super Paramagnetic Iron Oxide and Transfection

Abstract:

To explore the characteristics of magnetic resonance（MR）imaging of the rat endothelial progenitor cells（EPCs）labeled with superparamagnetic iron oxide（SPIO）. Total mononuclear cells (MNCs) were isolated from SD rat peripheral blood by ficoll density gradient centrifugation, and then the cells were plated on fibronectin-coated culture dishes. Attached cells were collected after 7 days cultured. EPCs were indentified by the laser confocal microscope and were counted in the inverted fluorescence microscope. EPCs were incubated with Fe2O3-arginine for 24 h, and the cells underwent MR imaging with three sequences (T1 WI, T2 WI, T2*WI). The results showed that the effective rate of labeled EPCs was 96%, and the survival rate of cells was 95%. The signal intensity on MRI was significantly decreased in labeled EPCs compared with unlabeled cells. EPCs labeled with SPIO can be sensitively displayed by the MR imaging.

• Periodical: Advanced Materials Research (Volume 683)
• Pages: 885-888
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.683.885
• Online since: April 2013
• Authors: Na Chang, Jun Zhang, Jun Shi Zhang
• Keywords: Endothelial Progenitor Cell, Magnetic Resonance Imaging (MRI), SPIO

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