Influence of Surface Zeta Potential on Adhesion of Chlorella sp. to Substratum Surfaces

Abstract:

Microalgae, in wet conditions, tend to grow on surfaces and form biofilms. The adhesion of microalgae to surfaces is very important for algal mass culture. The formation and development of microalgal biofims are in large denpend on the properties of cell surface, substratum surface and gowth medium. In this paper, the influence of substratum surface zeta potential on the microalgal biofilms was particularly investigated. We focused on a widely-used microalgal strain, the freshwater autotrophic Chlorella sp..The adhesion phenomena of Chlorella sp. to surfaces with different zeta potential were observed microscopically. It was found that Chlorella sp. adhered easily to the surface with a positive zeta potential and difficultly to the surface with a negative zeta potential. We concluded that the surface zeta potential had a greater influence on the adhesion of microalgal cells to substratum surfaces. Our findings have important implications for microalgae mass culture and harvesting.

• Periodical: Advanced Materials Research (Volumes 690-693)
• Pages: 1431-1434
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.690-693.1431
• Online since: May 2013
• Authors: Xin Ru Zhang, Ze Yi Jiang, Hao Yuan, Yuan Xiang Lu, Liang Chen, Ai Hui Chou, Hai Yan, Xin Xin Zhang
• Keywords: Adhesion, Biofilm, Microalgae, Surface Treatment, Surface Zeta Potential

References

[1] Amaro, H., M. , , A. , C., Guedes, F., X., Malcat. Advances and perspectives in using microalgae to produce biodiesel. Applied Energy, Vol. 88(2011), pp.3402-3410

DOI: 10.1016/j.apenergy.2010.12.014

[2] Chun-Yen Chen, Kuei-Ling Yeh, Rifka Aisyah, Duu-Jong Lee, Jo-Shu Chang. Cultivation, photobioreactor design andharvesting of microalgae for biodiesel production: A critical review. Bioresource Technology,Vol. 102 (2011), pp.71-78

DOI: 10.1016/j.biortech.2010.06.159

[3] Rodney M. Donlan. Biofilms Microbial life on Surfaces. Emerging infectious Diseases, Vol. 8 (2002), pp.881-890

DOI: 10.3201/eid0809.020063

[4] Y. Chisti: Biodiesel from microalgae. Biotechnol Adv, Vol. 25 (2007), pp.294-306

[5] Jian Cao,Wenqiao Yuan,Z. J. Peiet al. A Preliminary Study of the Effect of Surface Texture on Algae Cell Attachment for a Mechanical-Biological Energy Manufacturing System. Journal of Manufacturing Science and Engineering, Vol. 131(2009), p.64505

DOI: 10.1115/1.4000562

[6] Lihua Cheng, Lin Zhang, Huanlin Chen,Congjie Gao. Carbon dioxide removal from air by microalgae cultured in a membrane photobioreactor. Separation and Purification Technology, Vol. 50 (2006), pp.324-329

DOI: 10.1016/j.seppur.2005.12.006

[7] Michael A. Borowitzka. Commercial production of microalgae： ponds, tanks, tubes and fermenters. Journal of Biotechnology, Vol. 70(1999), pp.313-321

DOI: 10.1016/s0168-1656(99)00083-8

[8] Wassall, M.A., Embery, G. and Bag, J. The role of hydrophobicity in Streptococcus sanguis and Streptococcus salivarius adhesion to salivary fraction-coated hydroxyapatite. Colloids Surfaces B Biointerfaces , Vol. 5 (1995), pp.143-152

DOI: 10.1016/0927-7765(95)01211-z

[9] Yan Li, Ya Hui Gao, Xue Song Li et al. Influence of surface free energy on the adhesion of marine benthic diatom Nitzschia closterium MMDL533. Colloids and Surfaces B: Biointerfaces, Vol. 75 ( 2010), pp.550-556

DOI: 10.1016/j.colsurfb.2009.09.026

[10] Altan Ozkan,Halil Berberoglu. Adehesion of Cholorella Vulgaris on hydrophilic and hydrophobic surfaces[C]. Denver, Colorado, USA，(2011)

[11] Rolf Bos,Henny Van Der Mei C,Henk J. Busscher. Physico-chemistry of initial microbial adhesive interactions – its mechanisms and methods for study. FEMS Microbiology Reviews, Vol. 23 (1999), pp.179-230

DOI: 10.1016/s0168-6445(99)00004-2

[12] Jia, X., Yan, H., Wang, Z., He, H., Xu, Q., Wang, H., Yin, C., Liu, L. Carbon dioxide fixation by Chlorella sp. USTB-01 with a fermentor-helical combined photobioreactor. Front. Environ. Sci. Engin. China, Vol. 5 (2011), p.402–408

DOI: 10.1007/s11783-010-0223-0

[13] Andersen, R., A. 2005. Algal culturing techniques. Elsevier Academic Press