Bacterial Toxicity of Functionalized Polystyrene Latex Nanoparticles Toward Escherichia coli

Jumpei Miyazaki; Yuta Kuriyama; Akihisa Miyamoto; Hayato Tokumoto; Yasuhiro Konishi; Toshiyuki Nomura

doi:10.4028/www.scientific.net/AMR.699.672

Paper Titles

Liquid Metallurgy Synthesis and Thermo-Electrical Characterization of Copper-Aluminum Metal Matrix Composite
p.650

Studies on Temperature Variation and Angular Distortion in Submerged Arc Welded Butt Joint
p.656

Photocatalytic Splitting of Water on Cu-TiO₂ Nanotubes
p.662

Significance and Expression of Heat Shock Protein 72 and Glycoprotein96 in Human Gastroenterological Cancers
p.667

Bacterial Toxicity of Functionalized Polystyrene Latex Nanoparticles Toward Escherichia coli
p.672

Development and Antimicrobial Properties of a Composite Sorbent from Carbonized Rice Husk and Fugate of Sporogenous Bacteria
p.678

Theophylline Cocrystallized with Salicylic Acid and Picolinic Acid: Preparation and Physical Properties
p.682

Study on Syntheses and Anticoagulant Action of Rare Earth Ternary Complexes with Tryptophan and Sodium Citrate
p.689

Phytochemical Screening, Antioxidant Activity and Total Phenolic Content of Spirogyra spp.
p.693

HomeAdvanced Materials ResearchAdvanced Materials Research Vol. 699Bacterial Toxicity of Functionalized Polystyrene...

Bacterial Toxicity of Functionalized Polystyrene Latex Nanoparticles Toward Escherichia coli

Abstract:

Nanotechnology has the potential to produce a variety of new materials in the coming years, as a result of the design of novel nanoparticles with new physicochemical characteristics. However, their potential to adversely affect the environment and human health must be addressed. The toxicity of polystyrene latex (PSL) nanoparticles with various functional groups toward Escherichia coli KP7600 strain was investigated using the colony count method, and confocal microscopy observations. It was found that the positively charged PSL nanoparticles led to the death of the bacterial cells. Confocal observations of the bacterial cells after 1 h of exposure to the amine-modified, positively-charged PSL nanoparticles in an aqueous NaCl solution showed that the surfaces of the dead cells were almost entirely covered with the nanoparticles. No uptake of the nanoparticles into the bacterial cells was observed, regardless of the cell viability. It is likely that the adhesion of the positively charged nanoparticles onto the surface of the bacterial cells (due to the electrostatic attractive force) caused a decrease in the fluidity of the cell membrane, and the inhibition of metabolism through the cell membrane led to the death of the bacterial cells.